Molecular-based cross-species evaluation of bovine coronavirus infection in cattle, sheep and goats in Ghana
| Autor: | Christian Drosten, Samuel Oppong, Philip El-Duah, Yaw Oppong Frimpong, Richmond Yeboah, Augustina Sylverken, Vitus Burimuah, Ben Emikpe, Jones Lamptey, Olivia Agbenyega, RD Folitse, Yaw Adu-Sarkodie, William Tasiame, Eddie-Williams Owiredu, Michael Owusu, C. Antwi |
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| Rok vydání: | 2020 |
| Předmět: |
Diarrhea
Veterinary medicine Cattle Diseases Sheep Diseases Biology Ghana law.invention symbols.namesake law Bovine coronavirus Prevalence Animals Ovis Polymerase chain reaction Phylogeny Sanger sequencing Coronavirus Bovine Respiratory Distress Syndrome lcsh:Veterinary medicine Goat Diseases Sheep General Veterinary Base Sequence business.industry Reverse Transcriptase Polymerase Chain Reaction Goats Bayes Theorem General Medicine biology.organism_classification RNA-Dependent RNA Polymerase GenBank symbols Goat lcsh:SF600-1100 Livestock Cattle Sample collection Capra business Coronavirus Infections Cross-species infection Research Article |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 16, Iss 1, Pp 1-7 (2020) |
| ISSN: | 1746-6148 |
| Popis: | Background Apart from the huge worldwide economic losses often occasioned by bovine coronavirus (BCoV) to the livestock industry, particularly with respect to cattle rearing, continuous surveillance of the virus in cattle and small ruminants is essential in monitoring variations in the virus that could enhance host switching. In this study, we collected rectal swabs from a total of 1,498 cattle, sheep and goats. BCoV detection was based on reverse transcriptase polymerase chain reaction. Sanger sequencing of the partial RNA-dependent RNA polymerase (RdRp) region for postive samples were done and nucleotide sequences were compared with homologous sequences from the GenBank. Results The study reports a BCoV prevalence of 0.3%, consisting of 4 positive cases; 3 goats and 1 cattle. Less than 10% of all the animals sampled showed clinical signs such as diarrhea and respiratory distress except for high temperature which occurred in > 1000 of the animals. However, none of the 4 BCoV positive animals manifested any clinical signs of the infection at the time of sample collection. Bayesian majority-rule cladogram comparing partial and full length BCoV RdRp genes obtained in the study to data from the GenBank revealed that the sequences obtained from this study formed one large monophyletic group with those from different species and countries. The goat sequences were similar to each other and clustered within the same clade. No major variations were thus observed between our isolates and those from elsewhere. Conclusions Given that Ghana predominantly practices the extensive and semi-intensive systems of animal rearing, our study highlights the potential for spillover of BCoV to small ruminants in settings with mixed husbandry and limited separation between species. |
| Databáze: | OpenAIRE |
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