Detoxification of biomass hydrolysates with nucleophilic amino acids enhances alcoholic fermentation
| Autor: | Maobing Tu, Rui Xie, Jamarius Carvin, Yonnie Wu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Environmental Engineering Carboxylic acid Bioengineering 010402 general chemistry 7. Clean energy 01 natural sciences Hydrolysis 010608 biotechnology Organic chemistry Biomass Amino Acids Waste Management and Disposal Histidine chemistry.chemical_classification Ethanol Renewable Energy Sustainability and the Environment Chemistry Tryptophan Temperature General Medicine Hydrogen-Ion Concentration 0104 chemical sciences Amino acid Biochemistry Biofuels Glycine Fermentation Cysteine |
| Zdroj: | Bioresource Technology. 186:106-113 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2015.03.030 |
| Popis: | Carbonyl compounds generated in biomass pretreatment hinder the biochemical conversion of biomass hydrolysates to biofuels. A novel approach of detoxifying hydrolysates with amino acids for ethanol production was developed. Among the 20 amino acids assessed for their detoxification efficiency and nucleophilicity, cysteine was the most effective one. It increased both ethanol productivity and final yield of biomass hydrolysates from 0.18 (untreated) to 1.77 g/L/h and from 0.02 to 0.42 g/g, respectively. Detoxification efficiency was followed by histidine and it increased the final yield to 0.42 g/g, then by lysine, tryptophan and asparagine. It was observed all five effective amino acids contained reactive side-chain functional groups, which played important roles in the amino acid detoxification reaction. The study further showed cysteine and glycine detoxifications were temperature and pH dependent. The mechanistic study using mass spectrometry revealed thiazolidine carboxylic acid, a Schiff base, was formed by condensation of aldehyde and cysteine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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