An ELISA test for detecting chloroquine in urine
| Autor: | F.C. Shenton, M. Bots, T. A. Eggelte, A. Menon, Brian Greenwood, M. Y. L. De Wit |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.drug_class
Positive reaction Enzyme-Linked Immunosorbent Assay Urine Amodiaquine Pharmacology Monoclonal antibody Sensitivity and Specificity Chloroquine medicine Humans Chromatography biology Chemistry Public Health Environmental and Occupational Health Antibodies Monoclonal Cross reactions General Medicine Infectious Diseases Polyclonal antibodies Child Preschool Elisa test Aminoquinolines biology.protein Colorimetry Parasitology medicine.drug |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 82:216-220 |
| ISSN: | 0035-9203 |
| DOI: | 10.1016/0035-9203(88)90415-4 |
| Popis: | Two ELISA tests for detecting chloroquine in urine have been developed using polyclonal and monoclonal antibodies which react with the 7-chloro-4-aminoquinoline part of the chloroquine molecule and thus recognize chloroquine, its metabolites, and amodiaquine. The ELISAs were sensitive and specific and did not cross-react with other commonly used antimalarials. In a field trial the chloroquine ELISA performed better than the Dill Glazko or Haskins colorimetric tests. A small proportion of urines gave an apparently false positive reaction when tested at a dilution of 1:10, but not when tested at higher dilution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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