Prozone rates in the solid-phase platelet crossmatch assay and correlation with class I HLA antibody levels
Autor: | Laurie L. Wakefield, Kristie K. Clymer, Justin E. Juskewitch, Manish J. Gandhi, Justin D. Kreuter, Micah D. Zuccarelli |
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Rok vydání: | 2021 |
Předmět: |
medicine.medical_specialty
biology business.industry Mean fluorescence intensity Histocompatibility Testing Immunology Hematology Gastroenterology Antibodies Correlation Cross-Sectional Studies HLA Antigens Isoantibodies Internal medicine medicine biology.protein Immunology and Allergy Humans Hla antibodies Platelet crossmatch Sample dilution Antibody business |
Zdroj: | TransfusionREFERENCES. 61(11) |
ISSN: | 1537-2995 |
Popis: | Solid-phase platelet crossmatch (PXM) testing is used to help manage patients with platelet transfusion-refractoriness. Recently, we published the first report of false-negative PXM results from prozone effect that was mitigated using sample dilution. This study aimed to describe the prevalence of PXM prozone effect and the levels of class I HLA antibodies (HLA-Abs) associated with positive PXM results and with false-negative PXM results from prozone effect.A cross-sectional study of patients undergoing PXM testing from July 2019 through December 2020 was performed. All PXM tests were run simultaneously using undiluted and 1:4 diluted patient plasma. Prozone effect was defined as a negative PXM result using undiluted patient plasma but a positive PXM result using 1:4 diluted patient plasma.Among 59 patients, 830 individual ABO-compatible PXM results yielded an overall positivity rate of 25.8% (214/830) and a false-negative rate from prozone effect of 4.7% (10/214). Among the 28 patients with class I HLA-Ab testing and no other anti-platelet antibodies, maximum HLA-Ab mean fluorescence intensity (MFI) was significantly associated with a positive PXM result (p .0001; AUC approx. 0.9) and categorized into negative (3700), indeterminate (3700-10300), and positive (10300) maximum HLA-Ab MFI zones. Maximum HLA-Ab MFI, however, was not associated with prozone effect (p = .17; AUC approx. 0.6).While there is a strong predictive association between class I HLA-Ab levels and positive PXM results, PXM prozone effect is a common occurrence not associated with class I HLA-Ab levels, so additional testing with diluted samples should be considered. |
Databáze: | OpenAIRE |
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