CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
| Autor: | Wolfgang Harand, Robson Antônio de Souza, Marta Ribeiro Barbosa, José Jackson Pereira da Silva, Elizabete Cristina Pacheco dos Santos, Jean Philippe Guimarães Sauvé, Laureen Michelle Houllou |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Características do desenvolvimento in vitro
food.ingredient Callus formation fungi food and beverages Forestry Micropropagation Biology Azadirachta biology.organism_classification Organogêneses via calo food Callus Shoot Botany lcsh:SD1-669.5 Micropropagação Callus organogenesis Subculture (biology) lcsh:Forestry Cotyledon In vitro developmental characteristics Explant culture |
| Zdroj: | Revista Árvore v.39 n.3 2015 Revista Árvore Universidade Federal de Viçosa (UFV) instacron:SIF Revista Árvore, Vol 39, Iss 3, Pp 439-445 (2015) |
| Popis: | The study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation. |
| Databáze: | OpenAIRE |
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