Evaluation of tissue integration of injectable, cell‐laden hydrogels of cocultures of mesenchymal stem cells and articular chondrocytes with an ex vivo cartilage explant model
| Autor: | Johnny Lam, Hannah A. Pearce, Shail M. Mehta, Jason L. Guo, Yu Seon Kim, Antonios G. Mikos, Gerry L. Koons, Brandon T. Smith, Emma Watson, Adam M. Navara, K. Jane Grande-Allen |
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| Rok vydání: | 2021 |
| Předmět: |
Cartilage
Articular 0106 biological sciences 0301 basic medicine Cell Bioengineering macromolecular substances complex mixtures 01 natural sciences Applied Microbiology and Biotechnology Chondrocyte 03 medical and health sciences Chondrocytes 010608 biotechnology medicine Animals Tissue Engineering Chemistry Cartilage Mesenchymal stem cell technology industry and agriculture Cell Differentiation Hydrogels Mesenchymal Stem Cells Chondrogenesis Coculture Techniques Cell biology 030104 developmental biology medicine.anatomical_structure Self-healing hydrogels Rabbits Ex vivo Biotechnology Explant culture |
| Zdroj: | Biotechnology and Bioengineering. 118:2958-2966 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.27804 |
| Popis: | This study investigated the chondrogenic activity of encapsulated mesenchymal stem cells (MSCs) and articular chondrocytes (ACs) and its impact on the mechanical properties of injectable poly(N-isopropylacrylamide)-based dual-network hydrogels loaded with poly(L-lysine) (PLL). To this effect, an ex vivo study model was employed to assess the behavior of the injected hydrogels - specifically, their surface stiffness and integration strength with the surrounding cartilage. The highest chondrogenic activity was observed from AC-encapsulated hydrogels, while the effect of PLL on MSC chondrogenesis was not apparent from biochemical analyses. Mechanical testing showed that there were no significant differences in either surface stiffness or integration strength among the different study groups. Altogether, the results suggest that the ex vivo model can allow further understanding of the relationship between biochemical changes within the hydrogel and their impact on the hydrogel's mechanical properties. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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