Cloning of IRAK1 and its upregulation in symptomatic mandarin fish infected with ISKNV
| Autor: | Yongwen Luo, Shaoping Weng, Xiao-Qiang Yu, Zhi-Xin Yin, Wei-Jian Chen, Qingxia Lu, Wei He, Jianguo He, Chao-Zheng Zhang |
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| Rok vydání: | 2009 |
| Předmět: |
Molecular Sequence Data
Biophysics Spleen Biology Kidney IRAK1 Biochemistry Article NF-κB Cell Line Fish Diseases Downregulation and upregulation medicine Animals Humans ISKNV Amino Acid Sequence Cloning Molecular Receptor Molecular Biology Blood Cells Innate immune system Kinase NF-kappa B Cell Biology Molecular biology Perciformes Up-Regulation Interleukin-1 Receptor-Associated Kinases medicine.anatomical_structure Mandarin fish Signal transduction Infection Kidney necrosis |
| Zdroj: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2009.03.137 |
| Popis: | Interleukin-1 receptor activated kinases (IRAKs) play crucial roles in the Toll-like receptor (TLR) mediated signal transduction pathways that control host innate immune responses. Here we report the cloning of an IRAK1 cDNA (named ScIRAK1) from the mandarin fish. The predicted ScIRAK1 peptide contains a death domain and a serine/threonine-specific kinase domain. Quantitative RT-PCR showed that ScIRAK1 mRNA was primarily expressed in blood cells and posterior kidney. Seven days following infection with infectious spleen and kidney necrosis virus (ISKNV), the ScIRAK1 mRNA level was significantly higher in the blood cells of clinically symptomatic fish than in the blood cells of asymptomatic fish or control fish injected with phosphate-buffered saline. Additional experiments showed that overexpression of ScIRAK1 in the 293T cells could induce NF-kappaB activation. These results suggest that ScIRAK1 may play a role in the pathology of ISKNV infection in the mandarin fish. |
| Databáze: | OpenAIRE |
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