Effects of albumin/glutaraldehyde glue on healing of colonic anastomosis in rats
| Autor: | Efstathios Kotidis, Stamatis Angelopoulos, Savvas Symeonidis, Kalliopi Despoudi, Konstantinos Tsalis, Manousos George Pramateftakis, Aggeliki Cheva, Ioannis Mantzoros, Dimitrios Konstantaras, Nikolaos Antoniou, Orestis Ioannidis |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Colon Adhesions Urology Adhesion (medicine) Anastomotic Leak Anastomosis Colonic anastomosis 03 medical and health sciences chemistry.chemical_compound Hydroxyproline 0302 clinical medicine Adhesives Surgical Wound Dehiscence medicine Animals Humans Collagenases Rats Wistar Bioglue™ Fibroblast Bursting pressure Wound Healing business.industry Incidence Anastomosis Surgical Gastroenterology Albumin Proteins General Medicine Albumin/glutaraldehyde Basic Study medicine.disease Surgery Rats medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Models Animal Collagenase 030211 gastroenterology & hepatology Tissue Adhesives Glutaraldehyde Collagen Segmental resection business medicine.drug |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 2219-2840 |
| Popis: | Aim To evaluate the effect of local surgical adhesive glue (albumin/glutaraldehyde-Bioglue) on the healing of colonic anastomoses in rats. Methods Forty Albino-Wistar male rats were randomly divided into two groups, with two subgroups of ten animals each. In the control group, an end-to-end colonic anastomosis was performed after segmental resection. In the Bioglue group, the anastomosis was protected with extraluminar application of adhesive glue containing albumin and glutaraldehyde. Half of the rats were sacrificed on the fourth and the rest on the eighth postoperative day. Anastomoses were resected and macroscopically examined. Bursting pressures were calculated and histological features were graded. Other parameters of healing, such as hydroxyproline and collagenase concentrations, were evaluated. The experimental data were summarized and computed from the results of a one-way ANOVA. Fisher's exact test was applied to compare percentages. Results Bursting pressures, adhesion formation, inflammatory cell infiltration, and collagen deposition were significantly higher on the fourth postoperative day in the albumin/glutaraldehyde group than in the control group. Furthermore, albumin/glutaraldehyde significantly increased adhesion formation, inflammatory cell infiltration, neoangiogenesis, and collagen deposition on the eighth postoperative day. There was no difference in fibroblast activity or hydroxyproline and collagenase concentrations. Conclusion Albumin/glutaraldehyde, when applied on colonic anastomoses, promotes their healing in rats. Therefore, the application of protective local agents in colonic anastomoses leads to better outcomes. |
| Databáze: | OpenAIRE |
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