Expressions of Matrix Metalloproteinases 2, 7, and 9 in Carcinogenesis of Pancreatic Ductal Adenocarcinoma
| Autor: | Iwona Sidorkiewicz, Łukasz Lewczuk, Bogusław Kędra, Katarzyna Guzińska-Ustymowicz, Joanna Januszewska, Katarzyna Jakubowska, Andrzej Niewiński, Andrzej Kemona, Anna Pryczynicz |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Stromal cell Article Subject Carcinogenesis Clinical Biochemistry Inflammation Biology Adenocarcinoma medicine.disease_cause Neovascularization Immunoenzyme Techniques 03 medical and health sciences 0302 clinical medicine Stroma Genetics medicine Biomarkers Tumor Humans Molecular Biology lcsh:R5-920 Biochemistry (medical) Cancer General Medicine Middle Aged medicine.disease Prognosis Pancreatic Neoplasms 030104 developmental biology Matrix Metalloproteinase 9 030220 oncology & carcinogenesis Case-Control Studies Matrix Metalloproteinase 7 Cancer cell Matrix Metalloproteinase 2 Female medicine.symptom Neoplasm Grading Stromal Cells lcsh:Medicine (General) Carcinoma Pancreatic Ductal Follow-Up Studies Research Article |
| Zdroj: | Disease Markers, Vol 2016 (2016) Disease Markers |
| ISSN: | 1875-8630 0278-0240 |
| Popis: | Pancreatic ductal adenocarcinoma (PDAC) is a highly fatal disease, usually diagnosed in an advanced stage which gives a slight chance of recovery. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that participate in tissue remodeling and stimulate neovascularization and inflammatory response. The aim of the study was to evaluate the expression of MMP-2, MMP-7, and MMP-9 in normal ducts, tumor pancreatic adenocarcinoma cells, and peritumoral stroma in correlation with clinicohistopathological parameters. The study material was obtained from 29 patients with pancreatic ductal adenocarcinoma. The expressions of MMP-2, MMP-7, and MMP-9 were performed by immunohistochemical technique. Microvessel density (MVD) was visualized by special immunostaining. The expressions of MMP-2, MMP-7, and MMP-9 were mainly observed in tumor cells and peritumoral stroma. MMP-2 expression in cancer cells was correlated with female gender, stronger inflammation, and histopathological type of cancer (R=0.460,p=0.013;R=0.690,p=0.0001;R=-0.440,p=0.005, resp.). The expression of MMP-7 in tumor cells was found to positively correlate with the presence of necrosis and negatively correlate with MVD (R=0.402,p=0.031;R=-0.682,p=0.000). We also showed that positive MMP-9 expression in tumor cells was associated with MVD (R=0.368,p=0.084); however, it was not statistically significant. Our results demonstrate that MMP-2, MMP-7, and MMP-9 expressions correlate with various morphological features of the PDAC tumor such as inflammation, necrosis, and formation of the new blood vessels. |
| Databáze: | OpenAIRE |
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