Delayed Allergic Reaction to Suxamethonium Driven by Oligoclonal Th1-Skewed CD4+CCR4+IFN-γ+ Memory T Cells
| Autor: | E. C. Guerra, Adriano Mari, M. Giani, Enrico Scala, Maria Locanto, Chiara Mondino, L. Pirrotta |
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| Rok vydání: | 2006 |
| Předmět: |
Atropine
Receptors CCR4 Receptors Antigen T-Cell alpha-beta medicine.medical_treatment T cell Immunology Succinylcholine Biology Hysterectomy Skin Diseases Immunophenotyping Drug Hypersensitivity Interferon-gamma Postoperative Complications Antigen medicine Humans Immunology and Allergy Hypersensitivity Delayed Propofol Aged T-cell receptor General Medicine T lymphocyte T helper cell Th1 Cells Flow Cytometry Clone Cells medicine.anatomical_structure Cytokine Delayed hypersensitivity Neuromuscular Depolarizing Agents CD4 Antigens Female Receptors Chemokine Immunologic Memory Memory T cell Anesthetics Intravenous Adjuvants Anesthesia |
| Zdroj: | International Archives of Allergy and Immunology. 141:24-30 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000094178 |
| Popis: | Background: Muscle relaxants represent the drugs most frequently involved in intraoperative anaphylaxis during surgical procedures. Our aim was to report the case of a delayed reaction to suxamethonium and analyze specific T cell lines with regard to their specificity, phenotype and cytokine profile. Methods: We generated a drug-specific T cell line from a biopsy at the site of positive intradermal reactions and analyzed the immunophenotype, T cell receptor Vβ domain expression and cytokine profile. Results: T cells isolated from positive intradermal test reactions to suxamethonium showed a strict dose-dependent proliferation in response to drug-pulsed autologous antigen-presenting cells. The drug-specific CD4+ T cells were oligoclonal memory CD3+CD4+ T cells and expressed the skin homing receptors cutaneous lymphocyte antigen (CLA) and CCR4. Furthermore CD4+ suxamethonium-reactive T cell lines were IFN-γ-positive and synthesized high levels of IFN-γ and TNF-α. Conclusion: The study describes a delayed hypersensitivity to suxamethonium, driven by an oligoclonal T helper cell 1-skewed CD4+ memory T cell population, expressing the skin homing receptors CLA and CCR4. |
| Databáze: | OpenAIRE |
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