Expression of an active tobacco mitogen-activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize
| Autor: | Kan Wang, Joanne M. Yeakley, Jen Sheen, Jian-Bing Fan, Marina Bibikova, Patricia Bordallo, Huixia Shou |
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| Rok vydání: | 2004 |
| Předmět: |
Multidisciplinary
MAP kinase kinase kinase Kinase Acclimatization fungi food and beverages Biological Sciences Biology Mitogen-activated protein kinase kinase MAP Kinase Kinase Kinases Plants Genetically Modified biology.organism_classification Zea mays Electrolytes Biochemistry Freezing Tobacco Cold acclimation Carbohydrate Metabolism ASK1 Protein kinase A Transcription factor Nicotiana |
| Zdroj: | Proceedings of the National Academy of Sciences. 101:3298-3303 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0308095100 |
| Popis: | Cold acclimation is the major process that prepares plants for freezing tolerance. In addition to extensive transcription regulation by cold-inducible master transcription factors, oxidative stress signaling has been postulated to play a role in freezing tolerance. Activation of oxidative signaling through the expression of an active mitogen-activated protein kinase kinase kinase provided benefits in transgenic tobacco at freezing temperature bypassing cold acclimation. Because involvement of the mitogen-activated protein kinase cascade in oxidative stress signaling is evolutionarily conserved in eukaryotes from yeast to mammals, we tested the effect of expressing a heterologous tobacco mitogen-activated protein kinase kinase kinase ( Nicotiana PK1), which can mimic H 2 O 2 signaling, in a major cereal crop. We demonstrate that low-level but constitutive expression of the Nicotiana PK1 gene enhances freezing tolerance in transgenic maize plants that are normally frost sensitive. Our results suggest that a new molecular approach can be designed to genetically enhance freezing tolerance in important crops. |
| Databáze: | OpenAIRE |
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