An insight into the proteome of the saliva of the argasid tick Ornithodoros moubata reveals important differences in saliva protein composition between the sexes
| Autor: | Raúl Manzano-Román, L. Valero, A. Encinas-Grandes, Ricardo Pérez-Sánchez, Verónica Díaz-Martín, Ana Oleaga |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Proteomics Saliva Proteome Biophysics Tick Exosomes Biochemistry Microbiology Transcriptome Sex Factors stomatognathic system LC–MS/MS Tandem Mass Spectrometry Ornithodoros moubata Animals Secretion Ornithodoros Expressed Sequence Tags biology Protein equalization Computational Biology biology.organism_classification Blood meal Actins Recombinant Proteins Sialome Phosphopyruvate Hydratase Immunology Electrophoresis Polyacrylamide Gel Female Chromatography Liquid |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 26 páginas, 3 tablas, 6 figuras. -- The definitive version is available at http://www.elsevier.com Tick saliva contains pharmacologically active molecules that allow these parasites to obtain a blood meal from the host and facilitate host infection by tick-borne pathogens. Recent transcriptomic and proteomic analyses of the salivary glands of several tick species have provided data sets that are invaluable for a better understanding of tick sialomes and tick– host–pathogen relationships. Here we performed a proteomic study of the saliva from the argasid tick Ornithodoros moubata. Saliva samples from female and male specimens were analyzed separately by LC–MS/MS before and after their equalization to facilitate the identification of the less abundant proteins. We report the array of 193 proteins identified in the saliva of O. moubata showing: (i) the broad and complex composition of the saliva of this tick, in good agreement with the complexity of the argasid and ixodid sialomes described previously; (ii) a notable difference in the saliva proteomes of females and males, since only 10 of the proteins identified appeared to be shared by both sexes; and (iii) the presence in the salivary fluid of a wide range of proteins known to be housekeeping/intracellular, which could be secreted in unconventional ways, including exosome secretion. Financial support: Project AGL2010-18164, from Ministerio de Ciencia e Innovación,Spain; Project CSI062A11-2, from the Regional Government of Castilla y León, Spain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|