| Autor: |
Vijeta Rai, Sunil Venkanna Pogu, Rajat Bhatnagar, Pranish Bomzan, Ankita Dutta, Amitava Mandal, Mahendra Nath Roy, Anoop Kumar, Shilpi Ghosh |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Chemico-Biological Interactions. 360:109935 |
| ISSN: |
0009-2797 |
| Popis: |
Cancer remains a major world health issue due to its high morbidity and mortality rate. Plant based natural products (NPs) have played vital role in discovery of valuable anti-cancer drugs. Darjeeling Himalayan region has a rich diversity of therapeutic plants that can be utilized for development of novel drugs.We previously reported cytotoxic potential of rhizome extract of A.rivularis, a Darjeeling himalayan herb. Present study reports isolation and characterization of a phytosteroid from the plant rhizome in a bioassay-guided approach and evaluation of its anti-tumorigenic potential.The phytosteroid was characterized as stigmasta-5(6), 22(23)-dien-3-beta-yl acetate (A11) by various spectrometric techniques (IR, NMR, MS etc.). The catalytic inhibition and structural alteration of human dihydrofolate reductase (hDHFR) by A11 was evaluated using methotrexate (MTX), a DHFR inhibitor anticancer drug as a reference. A11 inhibited hDHFR activity with IC |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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