Flow cytometric detection of anti-AB antibody titers in blood group O recipients of blood group A2 donor kidneys
| Autor: | Pinar Ata, Leyla Ozel, T. Ozgezer, F. Cetinkaya, E. Eksioglu, Aysin Tulunay, Mesut İzzet Titiz |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors Hemagglutination Turkey Gastroenterology Antibodies Flow cytometry ABO Blood-Group System Young Adult Predictive Value of Tests Internal medicine medicine Humans Kidney transplantation Transplantation medicine.diagnostic_test biology business.industry Histocompatibility Testing Antibody titer Hemagglutination Tests medicine.disease Flow Cytometry Kidney Transplantation Histocompatibility Titer Treatment Outcome Blood Group Incompatibility Immunology biology.protein Surgery Female Antibody business |
| Zdroj: | Transplantation proceedings. 44(6) |
| ISSN: | 1873-2623 |
| Popis: | Aim ABO-incompatible kidney transplantation has been accepted for end-stage renal failure patients who have no ready opportunity for a deceased or living donor. Antibody titration for ABO-incompatible renal transplantation is not only difficult but also lacks conformity among laboratories. Herein we analyzed 20 living related renal transplant couples to detect recipient anti-A2 antibody using flow cytometric analysis. Materials and methods Patients were admitted to our center for renal transplantation between January 1999 and December 2010. All but four of them had undergone a previous renal transplantation from an ABO-compatible donor but experienced graft failure. All donor blood groups were subtyped by our blood bank using a lectin-based dilution assay. To detect recipient anti-A2 antibody titers we used a tube hemagglutination method. A/B antibody titer analysis by flow cytometry incubated serially diluted serum samples with donor erythrocytes. Each analysis was repeated three times over a 2-week period using an older and the last sera simultaneously. Results The 13 male and 7 female patients showed our overall mean age of 32 ± 12 years. All patients had panel-reactive antibody levels below 15%. The level of flow cytometric antibody titers did not vary upon repeated analysis (P = .01). When compared with the tube method there was a discrepancy of the level at which the antibody titer became negative. Discussion Flow cytometric antibody titration is a practical and rapid technique to determine the amount of anti-A2 antibody in renal recipients. |
| Databáze: | OpenAIRE |
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