Noninvasive Detection of Inflammatory Changes in White Adipose Tissue by Label-Free Raman Spectroscopy
| Autor: | Madeline Leonard, Clifford A. Hudis, Dilip Giri, Xi Kathy Zhou, Andrew J. Dannenberg, Jose O. Aleman, Abigail S. Haka, Cassidy Carpenter, Neil M. Iyengar, Erika Sue, Jan L. Breslow, Jeanne M. Walker, Joshua Sterling, Daniel S. Gareau, Chi Zhang, Monica Morrow, Peter R. Holt, Priya Bhardwaj, Maryem Manzoor, Ishan Barman |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Adipose Tissue White Adipose tissue Inflammation White adipose tissue Disease Type 2 diabetes Spectrum Analysis Raman Article Analytical Chemistry Pathogenesis 03 medical and health sciences Mice medicine Animals Humans Obesity Label free Chemistry Cancer medicine.disease Mice Inbred C57BL Disease Models Animal 030104 developmental biology Female medicine.symptom |
| Zdroj: | Analytical chemistry. 88(4) |
| ISSN: | 1520-6882 |
| Popis: | White adipose tissue inflammation (WATi) has been linked to the pathogenesis of obesity-related diseases, including type 2 diabetes, cardiovascular disease, and cancer. In addition to the obese, a substantial number of normal and overweight individuals harbor WATi, putting them at increased risk for disease. We report the first technique that has the potential to detect WATi noninvasively. Here, we used Raman spectroscopy to detect WATi with excellent accuracy in both murine and human tissues. This is a potentially significant advance over current histopathological techniques for the detection of WATi, which rely on tissue excision and, therefore, are not practical for assessing disease risk in the absence of other identifying factors. Importantly, we show that noninvasive Raman spectroscopy can diagnose WATi in mice. Taken together, these results demonstrate the potential of Raman spectroscopy to provide objective risk assessment for future cardiometabolic complications in both normal weight and overweight/obese individuals. |
| Databáze: | OpenAIRE |
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