Low Interferon Relative-Response to Cytomegalovirus Is Associated with Low Likelihood of Intrauterine Transmission of the Virus

Autor: Miriam Hillel, Maskit Bar-Meir, Eti Broide, G Abitbol, Yechiel Schlesinger, Yifat Eldar-Yedidia, Roni Falk, Marc V. Assous
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Cytomegalovirus Infection
0301 basic medicine
Human cytomegalovirus
Viral Diseases
Physiology
Cytomegalovirus
lcsh:Medicine
CD8-Positive T-Lymphocytes
Pathology and Laboratory Medicine
White Blood Cells
0302 clinical medicine
Animal Cells
Pregnancy
Interferon
Medicine and Health Sciences
Interferon gamma
Cell Cycle and Cell Division
030212 general & internal medicine
lcsh:Science
Immune Response
Likelihood Functions
Multidisciplinary
T Cells
virus diseases
Hematology
Prognosis
Body Fluids
Actinobacteria
Infectious Diseases
Blood
Cell Processes
Medical Microbiology
Viral Pathogens
Viruses
Cytomegalovirus Infections
Human Cytomegalovirus
Female
Cellular Types
Anatomy
Pathogens
Research Article
medicine.drug
Adult
Herpesviruses
Immune Cells
Immunology
Primary Cell Culture
Congenital cytomegalovirus infection
Biology
Microbiology
Virus
Interferon-gamma
Viral Proteins
03 medical and health sciences
Fetus
Immune system
medicine
Humans
Phytohemagglutinins
Microbial Pathogens
Secretion
Blood Cells
Bacteria
Uterus
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
medicine.disease
Virology
Infectious Disease Transmission
Vertical
030104 developmental biology
ROC Curve
lcsh:Q
Mitogens
DNA viruses
Physiological Processes
Mycobacterium Tuberculosis
Biomarkers
Zdroj: PLoS ONE, Vol 11, Iss 2, p e0147883 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: Background Congenital Cytomegalovirus (CMV) is a very common intrauterine infection which can cause severe mental and hearing impairments. Notably, only 40% of primarily infected women transmit CMV to the fetus. CMV-specific T-cell response has a role in CMV disease but individual immune heterogeneity precludes reliable correlation between measurable T-cells response and intrauterine transmission. Study Aim To establish a correlation between maternal T-cells response and fetal CMV transmission using an individual normalized immune response. Methods We analyzed IFN-γ secretion upon whole blood stimulation from primary CMV-infected pregnant women, with either CMV-peptides or PHA-mitogen. Results We established a new normalization method of individual IFN-γ response to CMV by defining the ratio between specific-CMV response and non-specific mitogen response (defined as IFN-γ relative response, RR), aiming to overcome high person-to-person immune variability. We found a unique subpopulation of women with low IFN-γ RR strongly correlated with absence of transmission. IFN-γ RR lower than 1.8% (threshold determined by ROC analysis) reduces the pre-test probability of transmission from 40% to 8%, revealing an unexpected link between low IFN-γ RR and non-transmission. Conclusion In pregnant women with primary CMV infection, low IFN-γ RR is associated with low risk of transmission.
Databáze: OpenAIRE
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