Bacillus xiapuensis sp. nov., isolated from marine sediment
| Autor: | Cetin Sengonca, Guo-Hong Liu, Jie-ping Wang, Jiang-Lin Lan, Qin-Ying Liu, Bo Liu, Haifeng Zhang, Jian-Mei Che |
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| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine China Geologic Sediments Bacillus Peptidoglycan Diaminopimelic Acid 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Cell Wall RNA Ribosomal 16S Seawater Food science Gene Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Bacillus (shape) Phosphatidylglycerol Base Composition biology Strain (chemistry) Phylogenetic tree Fatty Acids Nucleic Acid Hybridization Vitamin K 2 Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Terpenoid Bacterial Typing Techniques 030104 developmental biology chemistry |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 69:1714-1719 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003381 |
| Popis: | A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China. Growth was observed at 10–30 °C (optimum, 25 °C), in 0–7.0 % NaCl (0 %) and at pH 6.0–11.0 (pH 8.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7. The main fatty acids were anteiso-C17 : 0 (26.5 %), anteiso-C15 : 0 (19.6 %), iso-C15 : 0 (14.4 %) and C16 : 0 (10.5 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus , and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %). The average nucleotide identity and in silico DNA–DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon. The DNA G+C content was 44.2 mol%. The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp. nov. is proposed. The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T). |
| Databáze: | OpenAIRE |
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