Influences of Vagotomy on Gut Ischemia-Reperfusion Injury in Mice
| Autor: | Hiroshi Yasuhara, Taiki Miyakuni, Masashi Yanagawa, Satoshi Murakoshi, Motonari Ri, Yasuyuki Seto, Kazuhiko Fukatsu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Ischemia Vagotomy Critical Care and Intensive Care Medicine Gastroenterology 03 medical and health sciences Mice 0302 clinical medicine Cytokines metabolism Ileum Internal medicine medicine.artery Intestine Small medicine Animals Superior mesenteric artery Intestinal Mucosa Chemokine CCL2 business.industry Interleukin-6 Tumor Necrosis Factor-alpha Vagus Nerve medicine.disease SMA Intestines 030220 oncology & carcinogenesis Reperfusion Injury Emergency Medicine Cytokines business Reperfusion injury 030217 neurology & neurosurgery |
| Zdroj: | Shock (Augusta, Ga.). 47(5) |
| ISSN: | 1540-0514 |
| Popis: | How vagotomy affects host responses to gut ischemia-reperfusion (I/R) is unclear.Experiment 1: male Institute of Cancer Research mice (n = 22) were assigned to the I/R or the vago-I/R group. The I/R mice underwent 45-min superior mesenteric artery (SMA) occlusion. The vago-I/R mice received vagotomy before SMA occlusion. Survival was observed for 48 h.Experiment 2: mice (n = 55) were divided into four groups (Sham, vago, I/R, vago-I/R). Sham and vago groups did not undergo gut I/R. Mice were killed at 3 or 6 h after reperfusion, and cytokine levels in the plasma, jejunum, and ileum were evaluated. In addition, gut histology at 6 h was examined.Experiment 3: mice (n = 24) were divided into four groups as in Experiment 2. The small intestine was harvested at 3 h after reperfusion and the tissue was cultured ex vivo for 3 h. Cytokine levels of the culture supernatant were then measured.Experiment 1: survival was significantly worse with vago-I/R than I/R.Experiment 2: along with severe gut injury, vago-I/R increased IL-6 and monocyte chemoattractant protein-1 (MCP-1) in plasma, IFN-γ in the jejunum and MCP-1 in the ileum, as compared with I/R. Significant positive correlations were noted between plasma and intestinal levels of pro-inflammatory cytokines (IL-6, MCP-1, and TNF-α).Experiment 3: MCP-1 in the jejunal culture medium was higher in the vago-I/R than in the I/R group.Vagotomy worsens survival after gut I/R, together with increases in pro-inflammatory cytokines in both plasma and the gut in association with severe intestinal tissue damage. |
| Databáze: | OpenAIRE |
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