Detection of Two Anaerobic Periodontopathogens in Children by Means of the BANA and ELISA Assays
| Autor: | Walter J. Loesche, M.-R. Watson, Walter A. Bretz, Dennis E. Lopatin, I.J. Ertel |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
0301 basic medicine Adolescent Population Dental Plaque Enzyme-Linked Immunosorbent Assay Porphyromonas Dental plaque Benzoylarginine-2-Naphthylamide Microbiology 03 medical and health sciences 0302 clinical medicine stomatognathic system Periodontal disease medicine Bacteroides Humans Treponema Child education General Dentistry Periodontal Diseases Analysis of Variance education.field_of_study Chi-Square Distribution biology Hydrolysis Dental Plaque Index Treponema denticola 030206 dentistry biology.organism_classification medicine.disease Black or African American 030104 developmental biology Child Preschool Female Periodontal Index Anaerobic exercise |
| Zdroj: | Journal of Dental Research. 70:1052-1056 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | The mouths of young children become colonized by a variety of bacteria, but there have been only a few studies that have sought the presence of periodontopathic species in this population. Almost all of these studies used culturing techniques rather than the newer detection methodologies for various periodontopathogens. Studies in adults have shown that Treponema denticola and Porphyromonas (Bacteroides) gingivalis can be detected in dental plaque by use of the BANA and ELISA diagnostic tests. In the present study, plaque samples from four subgingival sites in each of 157 children (aged from two to 18 years) were tested for BANA hydrolysis with a BANA reagent card, and for T. denticola and P. gingivalis with an ELISA assay. Anaerobic periodontopathogens hydrolyzing the BANA substrate were found to be present in at least one of four plaque samples in 88 children (56%). T. denticola and/or P. gingivalis were detected by ELISA in at least one plaque sample in each of 135 children (86%). This study shows that children are widely colonized by these micro-organisms. A higher proportion of Black children than Caucasian children was colonized by these BANA-positive organisms. Also, children having a parent with a documented history of periodontal disease were more likely to be BANA-positive than were children of parents with unknown periodontal status. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|