Inhibition of chemotactic factor-induced neutrophil responsiveness by arachidonic acid
| Autor: | Paul H. Naccache, Elmer L. Becker, T.F.P. Molski, Ramadan I. Sha'afi, M. Volpi, W. M. Mackin |
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| Rok vydání: | 1983 |
| Předmět: |
Agonist
Cell Membrane Permeability Neutrophils Physiology medicine.drug_class Sodium Clinical Biochemistry chemistry.chemical_element Arachidonic Acids Calcium Calcium in biology chemistry.chemical_compound Methionine Extracellular medicine Animals Drug Interactions Neutrophil aggregation Cell Aggregation Arachidonic Acid N-Formylmethionine Dose-Response Relationship Drug Cell Membrane Chemotaxis Cell Biology N-Formylmethionine Leucyl-Phenylalanine Biochemistry chemistry Arachidonic acid Rabbits Oligopeptides |
| Zdroj: | Journal of Cellular Physiology. 115:243-248 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.1041150305 |
| Popis: | Arachidonic acid when added simultaneously with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (f-Met-Leu-Phe) inhibits the ability of the latter to initiate several but not all of its effects on rabbit peritoneal neutrophils. Stimulated neutrophil aggregation, calcium uptake, and increases in the steady state level of exchangeable calcium are all inhibited by 1-10 microM arachidonic acid. The binding of f-Met-Leu-Phe and the parameters of intracellular calcium redistribution (calcium efflux and changes in the steady state level of exchangeable calcium in the absence of extracellular calcium) and of stimulated sodium uptake are, on the other hand, unaffected by the same concentrations of arachidonic acid. Arachidonic acid, the saturated analog of arachidonic acid, was found not to inhibit f-Met-Leu-Phe-stimulated aggregation and calcium uptake. Arachidonic acid, therefore, in addition to its well-described agonist properties, also possesses antagonist activities toward rabbit neutrophils. These results add a new level of complexity to the study of the role of arachidonic acid in cell activation. |
| Databáze: | OpenAIRE |
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