Bioelectronic sniffers for ethanol and acetaldehyde in breath air after drinking
| Autor: | Kohji Mitsubayashi, Hiroyuki Matsunaga, Genki Nishio, Yusuke Nakanishi, Satoshi Toda |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Alcohol Drinking Biomedical Engineering Biophysics Poison control Aldehyde dehydrogenase Alcohol Acetaldehyde Biosensing Techniques Sensitivity and Specificity chemistry.chemical_compound Electrochemistry Humans Organic chemistry Ethanol metabolism Ethanol Chromatography biology Reproducibility of Results Equipment Design General Medicine Aldehyde Dehydrogenase Alcohol oxidase Equipment Failure Analysis Alcohol Oxidoreductases Breath Tests Breath gas analysis chemistry Odorants biology.protein Electronics Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 20:1573-1579 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2004.08.007 |
| Popis: | Two kinds of bioelectronic gas sensors (bio-sniffer) incorporating alcohol oxidase (AOD) and aldehyde dehydrogenase (ALDH) were developed for the convenient analysis of ethanol and acetaldehyde in expired gas, respectively. The sniffer devices for gaseous ethanol and acetaldehyde were constructed by immobilizing enzyme on electrodes covered with filter paper and hydrophilic PTFE membrane, respectively. The AOD and ALDH sniffers were used in the gas phase to measure ethanol vapor from 1.0 to 500 ppm, and acetaldehyde from 0.11 to 10 ppm covering the concentration range encountered in breath after alcohol consumption. Both bio-sniffers displayed good gas selectivity which was attributed to the substrate specificity of the relevant enzymes (AOD and ALDH) as gas recognition material. From the results of physiological application, the bio-sniffers could monitor the concentration changes in breath ethanol and acetaldehyde after drinking. The ethanol and acetaldehyde concentrations in expired air from ALDH2 [-] (aldehyde dehydrogenase type 2 negative) subjects were higher than that of the ALDH2 [+] (positive) subjects. The results indicated that the lower activity of ALDH2 induced an adverse effect on ethanol metabolism, leading to ethanol and acetaldehyde remaining in the human body, even human expired air. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect