Group M-based HIV-1 Gag peptides are frequently targeted by T cells in chronically infected US and Zambian patients
| Autor: | Joseph Mulenga, Doug Ritter, Susan Allen, Ethan K. Gough, Paul A. Goepfert, Craig M. Wilson, Anju Bansal |
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| Rok vydání: | 2006 |
| Předmět: |
Male
T-Lymphocytes Immunology Zambia Peptide Enzyme-Linked Immunosorbent Assay HIV Infections Virus Immunopathology Immunology and Allergy Humans Typing Sida chemistry.chemical_classification AIDS Vaccines Immunity Cellular biology Group-specific antigen biology.organism_classification Virology Genes gag United States Infectious Diseases chemistry Lentivirus HIV-1 Female Viral disease |
| Zdroj: | AIDS (London, England). 20(3) |
| ISSN: | 0269-9370 |
| Popis: | BACKGROUND The enormous sequence diversity of HIV-1 has been a major obstacle in the development of a globally useful vaccine for AIDS. The consensus and ancestral sequence-based immunogens minimize the genetic distance between contemporary isolates and vaccine strains. Hence these sequences may be promising candidates for HIV vaccines or serve as a universal reagent set for evaluating Gag-specific responses. METHODS In this study, we measured the T-cell reactivity to consensus (subtype A, B, C and group M), ancestral (group M and subtype B) and HXB2 Gag peptides (15-mers overlapping by 11) in HIV-1-infected subjects from two reference populations. We evaluated the Gag-specific T-cell responses in 43 chronically infected US (subtype B) and 13 Zambian (subtype C) subjects using an interferon-gamma enzyme-linked immunosorbent spot assay. RESULTS Our findings demonstrate a broad cross-reactivity of nearly 70% among all the seven Gag immunogens evaluated. Consensus M sequences elicited similar levels of responses as did the consensus B, ancestral subtype B and HXB2 peptides in subtype B-infected US patients. In subtype C-infected Zambian subjects, responses of similar breadth and magnitude were elicited by consensus C, consensus M and ancestral M peptides. CONCLUSION Our data demonstrate that peptide pools based on consensus or ancestral M-based sequences can be used to evaluate Gag-specific responses elicited by subtype B or subtype C-based immunogens. |
| Databáze: | OpenAIRE |
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