Molecular cloning of a cotton phosphatase gene and its functional characterization
| Autor: | Ai Min Wu, Shi You Lu, M. A. Jenks, Guang Rong Zhao, Shuping Zhang, Jinyuan Liu |
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| Rok vydání: | 2010 |
| Předmět: |
Gossypium
Base Sequence Molecular Sequence Data Phosphatase Mutant Temperature General Medicine Hydrogen-Ion Concentration Sodium Chloride Biology Molecular cloning Biochemistry Molecular biology Phosphoric Monoester Hydrolases Recombinant Proteins Yeast chemistry.chemical_compound chemistry Nucleotidases Nucleotidase Gene expression Inositol Cloning Molecular Southern blot |
| Zdroj: | Biochemistry (Moscow). 75:85-94 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1134/s0006297910010116 |
| Popis: | In this study, we report isolation of a phosphatase gene designated GhHL1 from cotton and its functional characterization. GhHL1 transcripts were detected in all cotton tissues examined. Southern blotting analysis indicated that it exists in multiple-copies. Biochemical analysis showed that GhHL1 was Mg2+-dependent and cation-sensitive. Purified recombinant GhHL1 protein dephosphorylated both 3',5'-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3',5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities. Overexpression of GhHL1 complemented yeast hal2 mutant and enhanced yeast growth under elevated NaCl or LiCl, showing a role in salt tolerance associated with ionic stress response. Taken together, these results show that GhHL1 is a functional and good candidate gene, which might be used to improve salt tolerance in plants. |
| Databáze: | OpenAIRE |
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