The application of next-generation sequence-based DNA barcoding for bloodmeal detection in host-seeking wild-caught Ixodes scapularis nymphs
| Autor: | S. Dolynskyj, L. R. Lindsay, Genevieve Lumsden, Evgeny Zakharov, Claire M. Jardine, J. S. Weese |
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| Rok vydání: | 2021 |
| Předmět: |
Nymph
0106 biological sciences Host identification 030231 tropical medicine lcsh:Medicine Zoology 010603 evolutionary biology 01 natural sciences DNA barcoding Blacklegged tick General Biochemistry Genetics and Molecular Biology DNA sequencing Bloodmeal analysis 03 medical and health sciences 0302 clinical medicine parasitic diseases Animals DNA Barcoding Taxonomic Humans lcsh:Science (General) lcsh:QH301-705.5 Larva Sciurus carolinensis Ixodes biology Host (biology) lcsh:R Cytochrome c oxidase subunit I DNA General Medicine biology.organism_classification Research Note lcsh:Biology (General) Ixodes scapularis Vertebrates Next-generation sequencing Cattle lcsh:Q1-390 |
| Zdroj: | BMC Research Notes BMC Research Notes, Vol 14, Iss 1, Pp 1-7 (2021) |
| ISSN: | 1756-0500 |
| DOI: | 10.1186/s13104-021-05481-3 |
| Popis: | Objective Our objective was to apply next-generation sequence-based DNA barcoding to identify the remnant larval bloodmeals in wild-caught host-seeking (unengorged) Ixodes scapularis nymphs (n = 216). To infer host species identification, vertebrate DNA was amplified using universal primers for cytochrome c oxidase subunit I (COI) and sequenced using next-generation sequencing (NGS) for comparison against known barcode references. Results Bloodmeal identification was unsuccessful in most samples (99% of 216 specimens) demonstrating a very low detection rate of this assay. Sequences that surpassed quality thresholds were obtained for 41.7% of nymphs (n = 90) and of those, confident species identification was obtained for 15.6% of nymphs (n = 14). Wild host identifications were only obtained from 2 specimens, where DNA from the eastern grey squirrel (Sciurus carolinensis) was identified. Human and bovine DNA was identified in remaining nymphs and considered to be contaminants. Further optimization of the technique is required to improve detection of remnant bloodmeals in host-seeking nymphs. |
| Databáze: | OpenAIRE |
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