Morphological alterations in tongue epithelial cells infected by SARS‐CoV‐2: A case–control study
| Autor: | Eduardo Muniz Barretto Tinoco, Fábio Ramôa Pires, Roy H. Stevens, Barbara Bruno Fagundes Marques, Ricardo Guimarães Fischer, Justine Monteiro Monnerat Tinoco, Taísa Coelho Guimarães, Josué da Costa Lima Junior |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty RT‐PCR Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein SARS‐CoV‐2 Tongue medicine Humans In patient General Dentistry SARS-CoV-2 business.industry Case-control study COVID-19 Spike Protein Epithelial Cells Original Articles medicine.anatomical_structure Real-time polymerase chain reaction Otorhinolaryngology Case-Control Studies Immunohistochemistry Original Article business Brazil |
| Zdroj: | Oral Diseases |
| ISSN: | 1601-0825 1354-523X |
| DOI: | 10.1111/odi.13988 |
| Popis: | Objective The aim of the present case–control study was to evaluate the morphological aspects of the epithelial cells from the dorsum of the tongue and the expression of the SARS‐CoV‐2 Spike protein in these cells, in patients with and without COVID‐19 infection. Methods 24 individuals with at least one symptom of COVID‐19 were recruited among inpatients from Hospital Universitário Pedro Ernesto (Rio de Janeiro, Brazil). 14 patients who tested positive for COVID‐19 by RT‐PCR were included in the case group, and 10 patients who tested negative were included in the control group. Cytological smears from the dorsum of the tongue were obtained from all patients and analyzed using immunohistochemistry directed against SARS‐CoV‐2‐Spike protein. Morphological changes in epithelial cells were analyzed using light microscopy. Results Immunohistochemistry showed that 71% of the COVID‐19 patients presented epithelial cells positive for the presence of the SARS‐CoV‐2 Spike protein, and all cells coming from patients in the control group were negative. Cytological analysis showed significant differences when comparing epithelial cells from COVID‐19‐positive and COVID‐19‐negative patients. Conclusion COVID‐19 may generate dimensional changes in tongue epithelial cells; however, further studies are necessary to understand how this happens. |
| Databáze: | OpenAIRE |
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