Intraocular transplants of a human gastrinoma in immuno-suppressed rats: morphological, chromatographic and functional studies
| Autor: | Lars E. Ericson, Annica Dahlström, Håkan Ahlman, Ola Nilsson, Elvar Theodorsson, Birgitta Ryberg |
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| Rok vydání: | 1989 |
| Předmět: |
Male
medicine.medical_specialty Pathology Adolescent Physiology Anterior Chamber Clinical Biochemistry Radioimmunoassay Peptide hormone Biology Biochemistry Gastric Acid Cellular and Molecular Neuroscience Endocrinology Cyclosporin a Internal medicine Gastrins medicine Animals Humans Protein Precursors Gastrin Cholecystokinin Immunosuppression Therapy Gastrinoma Chromatography digestive oral and skin physiology Rats Inbred Strains medicine.disease Zollinger-Ellison syndrome Rats Transplantation Pancreatic Neoplasms Microscopy Electron Chromatography Gel Gastric acid Female hormones hormone substitutes and hormone antagonists Neoplasm Transplantation |
| Zdroj: | Regulatory peptides. 24(1) |
| ISSN: | 0167-0115 |
| Popis: | Tissue pieces of a metastatic human gastrinoma (ultrastructural Type II) were successfully transplanted to the anterior eye-chamber of rats immunosuppressed with Cyclosporin A. Immunocytochemical investigation of the transplants showed evidence for preserved endocrine activity of tumour cells with immunoreactivity towards the C-terminal of the gastrin/cholecystokinin molecule. Studies of gastric acid secretion in tumour-bearing rats and sham-operated controls with chronic gastric fistulas showed that the basal acid output did not differ between the groups during 3 weeks of study. However, the stimulated gastric acid secretion decreased after 5 days in both groups to remain significantly depressed throughout the study, an effect probably due to Cyclosporin A treatment of the groups. The concentration of immunoreactive gastrin in plasma from rats with tumours in oculo was 5 times higher than in sham-operated rats. Gastrin-34 was the major immunoreactive component in both patient serum and rat plasma. An immunoreactive fraction corresponding to component I was found in the patient serum, but not in the rat plasma, although present in the chamber fluid. Components corresponding to gastrin-17 were found both in the patient serum and in the rat plasma. The chromatographic pattern of the tumour was similar to that in rat chamber fluid. The dominating component corresponded to gastrin-17, while gastrin-34 represented the quantitatively smaller component. Gastrin-34 was, however, relatively more abundant in the tumour extract than in the chamber fluid. The study also indicates that a gastrin-producing tumour transplanted in oculo in immunosuppressed rats may increase the rat plasma concentration of the same molecular forms of gastrin as seen in the clinical situation. |
| Databáze: | OpenAIRE |
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