Inhibition of NF-κB promotes autophagy via JNK signaling pathway in porcine granulosa cells
| Autor: | Ihtesham Ul Haq, Shenming Zeng, Hui Gao, Lu Lin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system Cytoplasm Pyrrolidines MAP Kinase Kinase 4 Swine Biophysics Apoptosis Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound Pyrrolidine dithiocarbamate Ovarian Follicle Thiocarbamates medicine Autophagy In Situ Nick-End Labeling Animals Ovarian follicle Molecular Biology Transcription factor Progesterone Cell Proliferation Cell Nucleus Granulosa Cells c-jun NF-kappa B Transcription Factor RelA NF-κB Cell Biology Progesterone secretion Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Microscopy Fluorescence Cancer research Female Signal transduction Follicle Stimulating Hormone Apoptosis Regulatory Proteins Signal Transduction |
| Zdroj: | Biochemical and biophysical research communications. 473(1) |
| ISSN: | 1090-2104 |
| Popis: | The transcription factor nuclear factor-κB (NF-κB) plays an important role in diverse processes, including cell proliferation and differentiation, apoptosis and inflammation. However, the role of NF-κB in porcine follicle development is not clearly elucidated. In this study, we demonstrated that follicle stimulating hormone (FSH) increased the level of inhibitor of NF-κB (IκB) protein and promoted the cytoplasmic localization of p65, indicating that FSH inhibits the activation of NF-κB in porcine granulosa cells. Moreover, inhibition of NF-κB by FSH or another specific inhibitor of NF-κB, pyrrolidine dithiocarbamate (PDTC), could activate JNK signaling and enhance autophagic activity in porcine granulosa cells. Knockdown of RelA (p65) Subunit of NF-κB by RNA interference abrogated the activation of JNK signaling pathway and the increase of autophagic protein expression by FSH. Meanwhile, the functional significance of FSH or PDTC-mediated autophagy were further investigated. Our results demonstrated that the increased autophagy promoted progesterone secretion in porcine granulosa cells. Blockage of autophagy by chloroquine obviated the FSH or PDTC-induced progesterone production. Taken together, these results indicate that inhibition of NF-κB increased autophagy via JNK signaling, and promote steroidogenesis in porcine granulosa cells. Our results provide new insights into the regulation and function of autophagy in mammalian follicle development. |
| Databáze: | OpenAIRE |
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