Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
| Autor: | Vladimir Pinheiro do Nascimento, Daniela Tonini da Rocha, Hamilton Luiz de Souza Moraes, Felipe de Oliveira Salle, Carlos Tadeu Pippi Salle, Thales Quedi Furian, Karen Apellanis Borges |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
animal structures
Patogenicidade Virulence medicine.disease_cause Microbiology Antimicrobial susceptibility Resistência a antimicrobianos Pathogenic Escherichia coli Virology medicine Escherichia coli Animals Humans Pathogenicity Escherichia coli Infections Phylogeny Phylogenetic tree biology Bird Diseases APEC General Medicine Escherichia coli uropatogênica (UPEC) Amoxicillin biology.organism_classification Antimicrobial Escherichia coli patogênia aviária (APEC) Multiple drug resistance Infectious Diseases Filogenia Urinary Tract Infections Parasitology UPEC Chickens Bacteria Phylogenetic groups medicine.drug |
| Zdroj: | Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
| Popis: | Introduction: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible for avian colibacillosis and human urinary tract infections, respectively. There are genetic similarities between the APEC and UPEC pathotypes, suggesting the APEC strains could be a potential reservoir of virulence and antimicrobial-resistance genes for the UPEC strains. This study aimed to characterize and compare APEC and UPEC strains regarding the phylogroup classification, pathogenicity and antimicrobial susceptibility. Methodology: A total of 238 APEC and 184 UPEC strains were selected and characterized. The strains were assayed for antimicrobial susceptibility and classified into phylogenetic groups using a multiplex-PCR protocol. In addition, the APEC strains had previously been classified according to their in vivo pathogenicity. Results: The results showed that both pathotypes had variation in their susceptibility to most of the antimicrobial agents evaluated, with few strains classified as multidrug resistant. The highest resistance rate for both pathotypes was to amoxicillin. Classifying the APEC and UPEC strains into phylogenetic groups determined that the most frequently frequencies were for groups D and B2, respectively. These results reflect the pathogenic potential of these strains, as all the UPEC strains were isolated from unhealthy patients, and most of the APEC strains were previously classified as pathogenic. Conclusions: The results indicate that distribution into phylogenetic groups provided, in part, similar classification to those of in vivo pathogenicity index, as it was possible to adequately differentiate most of the pathogenic and commensal or low-pathogenicity bacteria. However, no relationship could be found between the specific antimicrobial agents and pathogenicity or phylogenetic group for either pathotype. |
| Databáze: | OpenAIRE |
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