Antimicrobial activity of Lactobacillus salivarius and Lactobacillus fermentum against Staphylococcus aureus
| Autor: | Hae-Soon Lim, Yvonne Achermann, Mi-Sun Kang, Mark E. Shirtliff, You-Jin Lim, Jong-Suk Oh, Karin Wuertz-Kozak, Janette M. Harro |
|---|---|
| Přispěvatelé: | University of Zurich, Achermann, Yvonne |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Limosilactobacillus fermentum Staphylococcus aureus Lactobacillus fermentum LysM 030106 microbiology 610 Medicine & health medicine.disease_cause 2726 Microbiology (medical) Microbiology 10234 Clinic for Infectious Diseases Antibacterial activity Biofilm Lactobacillus salivarius 03 medical and health sciences Bacterial Proteins 2400 General Immunology and Microbiology RNA Ribosomal 16S ddc:570 Lactobacillus Antibiosis medicine Humans Department Sport- und Gesundheitswissenschaften Immunology and Allergy Mouth Microbial Viability General Immunology and Microbiology biology Chemistry 2725 Infectious Diseases General Medicine biology.organism_classification Antimicrobial Anti-Bacterial Agents 3. Good health Infectious Diseases Biofilms 2723 Immunology and Allergy Ligilactobacillus salivarius Staphylococcus Peptidoglycan binding |
| Zdroj: | Pathogens and Disease, 75 (2) |
| ISSN: | 2049-632X |
| DOI: | 10.1093/femspd/ftx009 |
| Popis: | The increasing prevalence of methicillin-resistant Staphylococcus aureus has become a major public health threat. While lactobacilli were recently found useful in combating various pathogens, limited data exist on their therapeutic potential for S. aureus infections. The aim of this study was to determine whether Lactobacillus salivarius was able to produce bactericidal activities against S. aureus and to determine whether the inhibition was due to a generalized reduction in pH or due to secreted Lactobacillus product(s). We found an 8.6-log10 reduction of planktonic and a 6.3-log10 reduction of biofilm S. aureus. In contrast, the previously described anti-staphylococcal effects of L. fermentum only caused a 4.0-log10 reduction in planktonic S. aureus cells, with no effect on biofilm S. aureus cells. Killing of S. aureus was partially pH dependent, but independent of nutrient depletion. Cell-free supernatant that was pH neutralized and heat inactivated or proteinase K treated had significantly reduced killing of L. salivarius than with pH-neutralized supernatant alone. Proteomic analysis of the L. salivarius secretome identified a total of five secreted proteins including a LysM-containing peptidoglycan binding protein and a protein peptidase M23B. These proteins may represent potential novel anti-staphylococcal agents that could be effective against S. aureus biofilms. Pathogens and Disease, 75 (2) ISSN:2049-632X |
| Databáze: | OpenAIRE |
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