Further characterization of monoclonal antibodies to Echinococcus granulosus Antigen 5 and Antigen B
| Autor: | Michael D. Rickard, D. Liu, Marshall W. Lightowlers |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Sheep
Protease medicine.drug_class medicine.medical_treatment Antibodies Helminth Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay Biology biology.organism_classification Monoclonal antibody Molecular biology Epitope Echinococcus Epitopes Infectious Diseases Antigen Antigens Helminth Monoclonal medicine biology.protein Animals Parasitology Antibody Echinococcus granulosus |
| Zdroj: | International Journal for Parasitology. 22:391-394 |
| ISSN: | 0020-7519 |
| DOI: | 10.1016/s0020-7519(05)80019-0 |
| Popis: | Two monoclonal antibodies (24.14, 61A12) to Echinococcus granulosus Antigen 5 and two (31.15 and 39B3) to Antigen B were further characterized using modified sheep hydatid cyst fluid antigens (SHCF) in ELISA. None of these four monoclonals were directed against carbohydrate or lipid epitopes of SHCF antigens since they all reacted strongly with periodate or lipase-treated SHCF. On the other hand, they appeared to recognize SHCF determinants of protein nature as protease treatment of SHCF destroyed binding with the monoclonals. Anti-Antigen B monoclonals 31.15 and 39B3 showed strong reaction with boiled SHCF and anti-Antigen 5 monoclonal 24.14 did not. However, the second anti-Antigen 5 monoclonal 61A12 also reacted with boiled SHCF suggesting that some epitopes of Antigen 5 are heat stable. 24.14 and 61A12 may recognize a similar epitope of Antigen 5 whereas 39B3 may be against an epitope of Antigen B different from that recognized by 31.15. |
| Databáze: | OpenAIRE |
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