Spinal mechanisms of pudendal nerve stimulation-induced inhibition of bladder hypersensitivity in rats
| Autor: | Buffie Clodfelder-Miller, Xin Su, Jamie McNaught, Timothy J. Ness, Cary DeWitte |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Urinary Bladder
030232 urology & nephrology Methysergide Pain Stimulation Piperazines Article Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Phentolamine Cyclohexanes Neuromodulation Reflex medicine Animals Bladder Pain Urinary bladder Naloxone business.industry General Neuroscience Bicuculline Electric Stimulation Pudendal Nerve medicine.anatomical_structure Nociception Spinal Cord Anesthesia Female business 030217 neurology & neurosurgery Muscle Contraction medicine.drug |
| Zdroj: | Neuroscience Letters. 686:181-185 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2018.08.041 |
| Popis: | Bilateral electrical pudendal nerve stimulation (bPNS) reduces bladder hypersensitivity in rat models of bladder pain and anecdotally reduces pain in humans with pelvic pain of urologic origin. The spinal neurochemical mechanisms of this antinociception are unknown. In the present study, bladder hypersensitivity was produced by neonatal bladder inflammation in rat pups coupled with a second inflammatory insult as an adult. Visceromotor responses (VMRs; abdominal muscle contractions) to urinary bladder distension (UBD) were used as a nociceptive endpoint under urethane-isoflurane anesthesia. bPNS consisted of bilateral biphasic electrical stimulation of the mixed motor/sensory component of the pudendal nerves. Following determination of the inhibitory effect of bPNS on VMRs, pharmacological antagonists were administered via an intrathecal catheter onto the lumbosacral spinal cord and bPNS effects on VMRs redetermined. bPNS resulted in statistically significant inhibition of VMRs to UBD in hypersensitive rats that was statistically reduced by the intrathecal administration of methysergide, WAY100636, CGP35348 and strychnine but was unaffected by naloxone, bicuculline, phentolamine, ondansetron and normal saline. This study suggests that inhibitory effects of bPNS may include serotonergic, GABA-B-ergic and glycinergic mechanisms suggesting the potential for interaction of the neuromodulatory effect with concommitant drug therapies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect