Development of a recombinant nucleocapsid protein-based ELISA for the detection of IgM and IgG antibodies to SARS-CoV-2
| Autor: | Maryam Ranjbar, Marzieh Asadi, Marjan Nourigorji, Bahador Sarkari, Zohreh Mostafavi‐Pour, Kamiar Zomorodian, Zahra Shabaninejad, Mortaza Taheri‐Anganeh, Amir Maleksabet, Mohsen Moghadami, Amir Savardashtaki |
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| Rok vydání: | 2021 |
| Předmět: |
SARS-CoV-2
Process Chemistry and Technology Biomedical Engineering COVID-19 Bioengineering Enzyme-Linked Immunosorbent Assay General Medicine Nucleocapsid Proteins Applied Microbiology and Biotechnology Sensitivity and Specificity Recombinant Proteins Immunoglobulin M Immunoglobulin G Drug Discovery Molecular Medicine Humans Nucleocapsid Biotechnology |
| Zdroj: | Biotechnology and applied biochemistryREFERENCES. 69(6) |
| ISSN: | 1470-8744 |
| Popis: | Coronavirus 2019 (COVID-19) is a global concern for public health. Thus, early and accurate diagnosis is a critical step in management of this infectious disease. Currently, RT-PCR is routine diagnosis test for COVID-19, but it has some limitations and false negative results. enzyme-linked immunosorbent assay (ELISA) against SARS-CoV-2 antigens seems to be an appropriate approach for serodiagnosis of COVID-19. In the current study, an ELISA system, using a recombinant nucleocapsid (N) protein, was developed for the detection of IgM and IgG antibodies to SARS-CoV-2. The related protein was expressed, purified, and used in an ELISA system. Sera samples (67) for COVID-19 patients, as well as sera samples from healthy volunteers (112), along with sera samples from non-COVID-19 patients were examined by the ELISA system. The expression and purity of the recombinant N protein were approved by SDS-PAGE and Western blotting. The sensitivity of ELISA system was 91.04 and 92.53% for the detection of IgG and IgM antibodies, respectively. Moreover, the specificity of the developed ELISA system for IgG and IgM were 98.21 and 97.32%, respectively. Our developed ELISA system showed satisfactory sensitivity and specificity for the detection of antiSARS-CoV-2 IgM and IgG antibodies and could be used as a complementary approach for proper diagnosis of COVID-19. |
| Databáze: | OpenAIRE |
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