Double‐Beam Vibrating Mirror Flying Spot Scanning‐Integrating Microspectrophotometer

Autor:	James L. Engle, Jerome J. Freed
Rok vydání:	1968
Předmět:	Microscopy Materials science Field (physics) Ultraviolet Rays Plane (geometry) Scanning electron microscope business.industry computer.file_format Radiation Clone Cells Absorbance Radiation flux Optics Spectrophotometry Raster graphics business Instrumentation computer Beam (structure)
Zdroj:	Review of Scientific Instruments. 39:307-312
ISSN:	1089-7623 0034-6748
DOI:	10.1063/1.1683356
Popis:	Serial measurements of integrated ultraviolet absorbance of individual living cultured animal cells are possible with a double‐beam scanning microscope designed to maximize the signal‐to‐noise ratio obtained for a given radiation flux traversing the sensitive specimen. A 192‐line raster (2 sec/frame), scanned in the specimen plane by a focused spot of monochromatized radiation, is reconstructed by video technique to provide an image display and permit manual delineation of photometric fields of arbitrary shape, corresponding to the shape of the specimen. Analog circuits produce a read‐out proportional to the integrated absorbance present in the photometric field, unaffected by inhomogeneity in the distribution of absorbing material.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::939566a500a9d843b27967d95e73b603 https://doi.org/10.1063/1.1683356 Zobrazit plný text záznamu