Next generation sequencing and de novo transcriptome analysis of Costus pictus D. Don, a non-model plant with potent anti-diabetic properties

Autor: Sudha Narayana Rao, Sanchita Anand, Sreeja Gopinathan, Santosh Prasad Sarma, Sunjay Jude Fernandes, Raja C. Mugasimangalam, Vasanthan Jayakumar, Ramasamy S. Annadurai, Mohan A V S K Katta, Nandita Mullapudi, S. Murugesan
Rok vydání: 2012
Předmět:
de novo Assembly
Costus pictus
RNA-Seq
Transcriptome
chemistry.chemical_compound
Diabetes mellitus
Molecular signature
Abscisic acid
Chromatography
High Pressure Liquid
Genetics
biology
High-Throughput Nucleotide Sequencing
Bixin
PPAR agonist
Abscisic Acid (ABA)
Research Article
Biotechnology
medicine.drug
lcsh:QH426-470
Sequence analysis
lcsh:Biotechnology
Acyclic Monoterpenes
Molecular Sequence Data
Secondary metabolite
Genes
Plant
DNA sequencing
Next Generation Sequencing (NGS)
lcsh:TP248.13-248.65
medicine
Humans
Hypoglycemic Agents
High Performance Liquid Chromatography (HPLC)
Base Sequence
Plant Extracts
Terpenes
Computational Biology
Molecular Sequence Annotation
Costus
Methyltransferases
Sequence Analysis
DNA
biology.organism_classification
Carotenoids
Plant Leaves
lcsh:Genetics
Diabetes Mellitus
Type 2
chemistry
Monoterpenes
Abscisic Acid
Phytotherapy
Zdroj: BMC Genomics
BMC Genomics, Vol 13, Iss 1, p 663 (2012)
ISSN: 1471-2164
DOI: 10.1186/1471-2164-13-663
Popis: BackgroundPhyto-remedies for diabetic control are popular among patients with Type II Diabetes mellitus (DM), in addition to other diabetic control measures. A number of plant species are known to possess diabetic control properties.Costus pictusD. Don is popularly known as “Insulin Plant” in Southern India whose leaves have been reported to increase insulin pools in blood plasma. Next Generation Sequencing is employed as a powerful tool for identifying molecular signatures in the transcriptome related to physiological functions of plant tissues. We sequenced the leaf transcriptome ofC. pictususing Illumina reversible dye terminator sequencing technology and used combination of bioinformatics tools for identifying transcripts related to anti-diabetic properties ofC. pictus.ResultsA total of 55,006 transcripts were identified, of which 69.15% transcripts could be annotated. We identified transcripts related to pathways of bixin biosynthesis and geraniol and geranial biosynthesis as major transcripts from the class of isoprenoid secondary metabolites and validated the presence of putative norbixin methyltransferase, a precursor of Bixin. The transcripts encoding these terpenoids are known to be Peroxisome Proliferator-Activated Receptor (PPAR) agonists and anti-glycation agents. Sequential extraction and High Performance Liquid Chromatography (HPLC) confirmed the presence of bixin inC. pictusmethanolic extracts. Another significant transcript identified in relation to anti-diabetic, anti-obesity and immuno-modulation is of Abscisic Acid biosynthetic pathway. We also report many other transcripts for the biosynthesis of antitumor, anti-oxidant and antimicrobial metabolites ofC. pictusleaves.ConclusionSolid molecular signatures (transcripts related to bixin, abscisic acid, and geranial and geraniol biosynthesis) for the anti-diabetic properties ofC. pictusleaves and vital clues related to the other phytochemical functions like antitumor, anti-oxidant, immuno-modulatory, anti-microbial and anti-malarial properties through the secondary metabolite pathway annotations are reported. The data provided will be of immense help to researchers working in the treatment of DM using herbal therapies.
Databáze: OpenAIRE
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