Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness
| Autor: | Nikolay M. Filipov, Rachel L. Dockman, Donald A. Harn, John J. Wagner, Thomas Norberg, Jessica M. Carpenter, Ryan S. Mote, Andrew J. Steinberger, Garret Suen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
permethrin Glycan Health Toxicology and Mutagenesis lcsh:Medicine gut microbiome Context (language use) Gastroenterology and Hepatology Butyrate Biology Gut flora Gulf war Article Microbiology in the medical area 03 medical and health sciences Mice 0302 clinical medicine Polysaccharides RNA Ribosomal 16S Gastroenterologi Mikrobiologi inom det medicinska området Animals Persian Gulf Syndrome Beneficial effects lacto-N-fucopentaose-III (LNFPIII) Feces 030304 developmental biology 0303 health sciences Lachnospiraceae lcsh:R Public Health Environmental and Occupational Health Amino Sugars biology.organism_classification Gastrointestinal Microbiome Gulf War Mice Inbred C57BL Immunology Gulf War Illness biology.protein 030217 neurology & neurosurgery gut inflammation and health pyridostigmine bromide |
| Zdroj: | International Journal of Environmental Research and Public Health Volume 17 Issue 19 International Journal of Environmental Research and Public Health, Vol 17, Iss 7081, p 7081 (2020) |
| ISSN: | 1660-4601 |
| DOI: | 10.3390/ijerph17197081 |
| Popis: | The microbiota&rsquo s influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut&ndash brain&ndash immune axis. This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model. Male C57BL/6J mice were administered pyridostigmine bromide (PB 0.7 mg/kg) and permethrin (PM 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 &mu g/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2× /week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total). Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration. Although PB/PM did not affect species composition on a global scale, it affected specific taxa in both short- and long-term settings. PB/PM elicited more prominent long-term effects, notably, on the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum. LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria. Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples LNFPIII was modulatory. Overall, significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment was beneficial. |
| Databáze: | OpenAIRE |
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