Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid
| Autor: | Tomáš Skalický, Francisco J. Ayala, Jan Votýpka, Eva Dobáková, Julius Lukeš, Pavel Flegontov, Dagmar Jirsová, Vyacheslav Yurchenko, Martina Tesařová, Richard J. Wheeler |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Trypanosoma cruzi Protozoan Proteins Flagellum Genome 03 medical and health sciences parasitic diseases Humans Flagellate Cytoskeleton Phylogeny Leishmania Whole genome sequencing Life Cycle Stages Multidisciplinary biology Gene Expression Profiling fungi Biological Sciences biology.organism_classification Cell biology 030104 developmental biology Membrane protein Flagella Trypanosoma Ultrastructure Genome Protozoan Cytostome |
| Zdroj: | Proceedings of the National Academy of Sciences. 114(44) |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Paratrypanosoma confusum is a monoxenous kinetoplastid flagellate that constitutes the most basal branch of the highly diverse parasitic trypanosomatids, which include human pathogens Trypanosoma and Leishmania. This makes Paratrypanosoma uniquely informative for the evolution of obligatory parasitism from free-living lifestyle and the evolution of human parasitism in some trypanosomatid lineages. It has typical promastigote morphology but also forms surface-attached haptomonads and amastigotes. Haptomonads form by attachment to a surface via a large bulge at the base of the flagellum, which is then remodeled into a thin attachment pad associated with flagellum shortening. Promastigotes and haptomonads multiply by binary division, and the progeny of a haptomonad can either remain attached or grow a flagellum and resume swimming. Whole genome sequencing and transcriptome profiling, in combination with analysis of the cell ultrastructure, reveal how the cell surface and metabolism are adapted to parasitism and how characteristic cytoskeletal features are conserved. Our data demonstrate that surface attachment by the flagellum and the flagellar pocket, a Leishmania-like flagellum attachment zone, and a Trypanosoma cruzi-like cytostome are ancestral features, while evolution of extant trypanosomatids, including the human parasites, is associated with genome streamlining and diversification of membrane proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|