Repair of rabbit duodenal mucosa after acid injury in vivo and in vitro
| Autor: | P Vattay, Feil W, Tacettin Sogukoglu, Michael Starlinger, Etienne Wenzl, R. Schiessel |
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| Rok vydání: | 1987 |
| Předmět: |
Time Factors
Necrosis Cell division Duodenum Hydrochloric acid In Vitro Techniques Biology chemistry.chemical_compound In vivo medicine Intestinal epithelial cell migration Animals Intestinal Mucosa Hepatology digestive oral and skin physiology Gastroenterology Hydrogen-Ion Concentration Molecular biology In vitro Microscopy Electron medicine.anatomical_structure Biochemistry chemistry Gastric Mucosa Duodenal mucosa Female Hydrochloric Acid Rabbits medicine.symptom Cell Division |
| Zdroj: | Gastroenterology. 92:1973-1986 |
| ISSN: | 0016-5085 |
| DOI: | 10.1016/0016-5085(87)90632-9 |
| Popis: | Epithelial repair after luminal acid exposure was studied in the rabbit duodenum in vivo and in vitro. Hydrochloric acid (200 mM, 30 min in vivo; 10 mM, 10 min in vitro) caused a uniform damage of the mucosa confined to the villi. After demarcation and detachment of the necrotic tissue the defect was bridged by the remaining viable epithelial cells, resulting in a reduction of villus height. This process of mucosal repair proceeded continuously, so that 9 h after acid damage only 33% of villi in the proximal duodenum and 41% in the distal duodenum remained not fully restored, at both luminal pH 7 and luminal pH 3. The difference is due to the higher acid susceptibility of the distal duodenum. In vitro, most of the mucosal surface was reconstituted 5 h after acid injury, at both luminal pH 7.4 and luminal pH 3. The potential difference declined and reversed after mucosal damage; restitution to original values was only observed at luminal pH 7 in vivo and luminal pH 7.4 in vitro. |
| Databáze: | OpenAIRE |
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