Evaluation of Marine Agarose Biomaterials for Tissue Engineering Applications
| Autor: | Miguel Alaminos, Agustín Janer, Olimpia Ortiz-Arrabal, Javier Fernández, Antonio Campos, David Sánchez-Porras, María José de Frutos, Emilio Esteban, Fernando Campos, Ainhoa Irastorza-Lorenzo |
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| Přispěvatelé: | [Irastorza-Lorenzo,A, Sánchez-Porras,D, Ortiz-Arrabal,O, Campos,A, Campos,F, Alaminos,M] Tissue Engineering Group, Department of Histology, University of Granada and Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain. [de Frutos,MJ, Esteban,E, Fernández,J, Janer,A] Hispanagar, SA, Burgos, Spain., This research was funded by grant IDI-20180052 (Agarmatriz), leaded by Hispanagar SA, Burgos, Spain, through CDTI, Ministry of Science and Innovation, Spain, Programa Operativo Plurirregional de Crecimiento Inteligente (CRIN), award no. AC17/00013 (NanoGSkin project) by ISCIII thorough AES 2017 within the EuroNanoMed framework, grant PE-0395-2019 from Consejería de Salud y Familias, Junta de Andalucía, Spain, and by the Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i) from Ministerio de Ciencia, Innovación y Universidades (Instituto de Salud Carlos III), grants FIS PI17/0391, and PI20/0317 (co-financed by Fondo Europeo de Desarrollo Regional ERDF-FEDER, European Union). |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Análisis de materiales
Human skin Biocompatible Materials 02 engineering and technology Chemicals and Drugs::Carbohydrates::Polysaccharides::Sepharose [Medical Subject Headings] Biomechanical properties lcsh:Chemistry Agaroseagarose chemistry.chemical_compound Tissue engineering Materiales biocompatibles Agarose Organisms::Eukaryota::Animals [Medical Subject Headings] Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats::Rats Wistar [Medical Subject Headings] Oral mucosa Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Elasticity::Elastic Modulus [Medical Subject Headings] lcsh:QH301-705.5 Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineering [Medical Subject Headings] Spectroscopy Cells Cultured Skin Tissue Scaffolds Sefarosa Anatomy::Cells::Connective Tissue Cells::Fibroblasts [Medical Subject Headings] Chemistry Sepharose Hydrogels General Medicine 021001 nanoscience & nanotechnology Healthy Volunteers Computer Science Applications Biomechanical Phenomena Anatomy::Integumentary System::Skin [Medical Subject Headings] medicine.anatomical_structure tissue engineering Self-healing hydrogels Biocompatibility 0210 nano-technology agarose biomaterials Chemicals and Drugs::Biomedical and Dental Materials::Biocompatible Materials [Medical Subject Headings] Cell Survival 0206 medical engineering Ingeniería tisular Catalysis Article Inorganic Chemistry Biomaterials biocompatibility In vivo Elastic Modulus Fenómenos biomecánicos Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Follow-Up Studies [Medical Subject Headings] Persons::Persons::Volunteers::Healthy Volunteers [Medical Subject Headings] medicine Animals Humans Physical and Theoretical Chemistry Rats Wistar Chemicals and Drugs::Complex Mixtures::Colloids::Gels::Hydrogels [Medical Subject Headings] Molecular Biology biomechanical properties Analytical Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Prostheses and Implants::Tissue Scaffolds [Medical Subject Headings] Organic Chemistry Histology Fibroblasts Seaweed 020601 biomedical engineering Rats Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats [Medical Subject Headings] lcsh:Biology (General) lcsh:QD1-999 Anatomy::Cells::Cells Cultured [Medical Subject Headings] Phenomena and Processes::Physical Phenomena::Biophysical Phenomena::Biomechanical Phenomena [Medical Subject Headings] Organisms::Organism Forms::Aquatic Organisms::Seaweed [Medical Subject Headings] Biomedical engineering Follow-Up Studies |
| Zdroj: | International Journal of Molecular Sciences Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Digibug. Repositorio Institucional de la Universidad de Granada instname Volume 22 Issue 4 International Journal of Molecular Sciences, Vol 22, Iss 1923, p 1923 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Five agarose types (D1LE, D2LE, LM, MS8 and D5) were evaluated in tissue engineering and compared for the first time using an array of analysis methods. Acellular and cellular constructs were generated from 0.3–3%, and their biomechanical properties, in vivo biocompatibility (as determined by LIVE/DEAD, WST-1 and DNA release, with n = 6 per sample) and in vivo biocompatibility (by hematological and biochemical analyses and histology, with n = 4 animals per agarose type) were analyzed. Results revealed that the biomechanical properties of each hydrogel were related to the agarose concentration (p < 0.001). Regarding the agarose type, the highest (p < 0.001) Young modulus, stress at fracture and break load were D1LE, D2LE and D5, whereas the strain at fracture was higher in D5 and MS8 at 3% (p < 0.05). All agaroses showed high biocompatibility on human skin cells, especially in indirect contact, with a correlation with agarose concentration (p = 0.0074 for LIVE/DEAD and p = 0.0014 for WST-1) and type, although cell function tended to decrease in direct contact with highly concentrated agaroses. All agaroses were safe in vivo, with no systemic effects as determined by hematological and biochemical analysis and histology of major organs. Locally, implants were partially encapsulated and a pro-regenerative response with abundant M2- type macrophages was found. In summary, we may state that all these agarose types can be safely used in tissue engineering and that the biomechanical properties and biocompatibility were strongly associated to the agarose concentration in the hydrogel and partially associated to the agarose type. These results open the door to the generation of specific agarose-based hydrogels for definite clinical applications such as the human skin, cornea or oral mucosa. Hispanagar SA, Burgos, Spain, through CDTI, Ministry of Science and Innovation, Spain, Programa Operativo Plurirregional de Crecimiento Inteligente (CRIN) IDI-20180052 ISCIII thorough AES AC17/00013 Junta de Andalucía PE-0395-2019 Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i) from Ministerio de Ciencia, Innovación y Universidades (Instituto de Salud Carlos III) FIS PI17/0391 Fondo Europeo de Desarrollo Regional ERDF-FEDER, European Union PI20/0317 |
| Databáze: | OpenAIRE |
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