Immunomonitoring of graft-versus-host minor histocompatibility antigen correlates with graft-versus-host disease and absence of relapse after graft

Autor: David Laurin, Martine Pernollet, Agnès Moine, Jean-Claude Bensa, Joel Plumas, Jean-Yves Cahn, Frédéric Garban, Dalil Hannani
Přispěvatelé: Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Bases moléculaires de la progression tumorale -Groupe de Recherche sur les Lymphomes, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR73, Laboratoires Immunocytologie et HLA, département d'Immunologie Cellulaire, EFS, Hematology, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), TheREx, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Département de cancérologie et d'hématologie, CHU Grenoble-Hôpital Michallon-CHU Grenoble-Hôpital Michallon
Rok vydání: 2009
Předmět:
MESH: Minor Histocompatibility Antigens
Male
MESH: Tissue Donors
Transplantation Conditioning
medicine.medical_treatment
Graft vs Host Disease
Disease
Hematopoietic stem cell transplantation
MESH: Peripheral Blood Stem Cell Transplantation
MESH: T-Lymphocyte Subsets
CD8-Positive T-Lymphocytes
0302 clinical medicine
immune system diseases
Recurrence
T-Lymphocyte Subsets
Minor histocompatibility antigen
Immunology and Allergy
MESH: Bone Marrow Transplantation
MESH: Transplantation Conditioning
Bone Marrow Transplantation
0303 health sciences
MESH: Middle Aged
ELISPOT
MESH: Enzyme-Linked Immunosorbent Assay
[SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology
Hematology
MESH: Transplantation
MESH: Follow-Up Studies
Middle Aged
MESH: CD8-Positive T-Lymphocytes
Tissue Donors
3. Good health
Leukemia
surgical procedures
operative
MESH: Young Adult
Hematologic Neoplasms
Female
MESH: Immunosuppressive Agents
Immunosuppressive Agents
Adult
Adolescent
MESH: Interferon-gamma
Immunology
MESH: Graft vs Host Disease
Enzyme-Linked Immunosorbent Assay
Graft vs Leukemia Effect
Minor Histocompatibility Antigens
03 medical and health sciences
Interferon-gamma
Young Adult
Antigen
medicine
MESH: Transplantation
Homologous
Humans
Transplantation
Homologous
030304 developmental biology
MESH: Adolescent
Peripheral Blood Stem Cell Transplantation
Transplantation
MESH: Humans
business.industry
MESH: Adult
MESH: Graft vs Leukemia Effect
medicine.disease
MESH: Male
Histocompatibility
MESH: Recurrence
Graft-versus-host disease
Feasibility Studies
business
MESH: Feasibility Studies
MESH: Female
030215 immunology
MESH: Hematologic Neoplasms
Follow-Up Studies
Zdroj: Transfusion
Transfusion, Wiley, 2010, 50 (2), pp.418-28. ⟨10.1111/j.1537-2995.2009.02440.x⟩
ISSN: 1537-2995
0041-1132
DOI: 10.1111/j.1537-2995.2009.02440.x⟩
Popis: International audience; BACKGROUND: After HLA-identical hematopoietic stem cell transplantation, minor histocompatibility (mH) antigen alloreactivity plays a dominant role in the development of graft-versus-host disease (GVHD) and graft versus leukemia (GVL). STUDY DESIGN AND METHODS: We have analyzed the mH alloreactivity (enzyme-linked immunospot [ELISpot] for interferon-gamma[IFN-gamma] assay) from 24 donor/recipient pairs over a period of 2 years of follow-up and correlated such alloreactivity with the development of GVHD or absence of relapse. Circulating specific T cells anti-mH with multimer HLA-peptides were also studied. RESULTS: We show by ELISpot IFN-gamma assay that alloreactivity during the first 3 months from donor versus recipient or donor versus mismatched identified mH antigens is associated with acute GVHD and GVL effect. In addition, we demonstrate that the donor-versus-recipient reactivity observed after the third month is highly associated with chronic GVHD and GVL (p = 0.0007). Finally, we show by multimer HLA-peptide assay that mH epitope-specific T cells present after 3 months are statistically related to the GVL effect. CONCLUSIONS: Our results provide a robust method to monitor mH antigen graft-versus-host reaction and suggest that current identified mH have predictive value on GVHD and GVL.
Databáze: OpenAIRE
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