Synergistic effects of proanthocyanidin, tri-calcium phosphate and fluoride on artificial root caries and dentine collagen
| Autor: | Stephen Kwan, Cynthia K.Y. Yiu, Michael Frances Burrow, Dora Chu, DJ Epasinghe, May Mei Lei |
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| Rok vydání: | 2016 |
| Předmět: |
Calcium Phosphates
Materials science Bioengineering 02 engineering and technology Biomaterials 03 medical and health sciences Hydroxyproline chemistry.chemical_compound Fluorides 0302 clinical medicine X-Ray Diffraction medicine Humans Proanthocyanidins Collagenases Composite material Root caries Remineralisation Minerals Microscopy Confocal Drug Synergism 030206 dentistry Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Phosphate Microradiography Demineralization chemistry Proanthocyanidin Mechanics of Materials Root Caries Dentin Collagenase Collagen 0210 nano-technology Crystallization Fluoride Nuclear chemistry medicine.drug |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 73 |
| ISSN: | 1873-0191 |
| Popis: | Background Proanthocyanidin has been shown to enhance dentine collagen stability and remineralization of artificial root caries. Objectives To evaluate the effect of proanthocyanidin (PA) in combination with tri-calcium phosphate (TCP) and fluoride (F) on resistance to collagen degradation and remineralization of artificial caries lesions. Methods Demineralized root fragments ( n = 75) were randomly divided into five groups based on treatments: (i) 6.5% PA, (ii) TCP + F, (iii) TCP + F + 6.5% PA, (iv) 1000 ppm fluoride (Positive control) and (v) deionized water (control). Each specimen was subjected to pH cycling at 37 o C for 8 days. Lesion depth and mineral loss were evaluated using microradiography and confocal laser scanning microscopy. The type of crystal formation was determined by XRD spectra. To evaluate the stability of root caries lesions against collagenase challenge, highly purified type VII collagenase from Clostridium was added to obtain a remineralizing solution that contained 7.5 U/mL collagenase and pH cycling was repeated. The different remineralizing solutions were collected after the pH cycling to assess the amount of hydroxyproline release. Collagen degradation depth and lesion depth were evaluated using transverse microradiography. Resistance to collagen degradation was determined using hydroxyproline assay. Data were analyzed using one-way ANOVA and Tukey multiple comparison tests. Results Results of one-way ANOVA showed that the test solutions had a significant effect on mineral loss ( p p p p p p Conclusion The addition of PA to TCP + F reduced collagen degradation, inhibited demineralization and enhanced remineralization. |
| Databáze: | OpenAIRE |
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