Genome-Wide Identification and Characterization of Actin-Depolymerizing Factor (ADF) Family Genes and Expression Analysis of Responses to Various Stresses in Zea Mays L

Autor: Wenbin Deng, Jun Huang, Shija Joseph, Xin Wang, Wei Sun, Ruichun Yang, Jingsheng Fan, Lihong Zhai, Yunfeng Li, Jiaxin Ren
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Arabidopsis
01 natural sciences
Genome
lcsh:Chemistry
Plant Growth Regulators
Gene Expression Regulation
Plant
lcsh:QH301-705.5
Phylogeny
Spectroscopy
Oligonucleotide Array Sequence Analysis
Genetics
biology
Phylogenetic tree
food and beverages
General Medicine
Zea Mays L
Droughts
Computer Science Applications
Actin depolymerizing factor
ADF genes
Pollen
Genome
Plant
macromolecular substances
Physcomitrella patens
Zea mays
Article
Chromosomes
Plant
Catalysis
Inorganic Chemistry
03 medical and health sciences
Stress
Physiological
Gene family
Physical and Theoretical Chemistry
Molecular Biology
Gene
Genetic Association Studies
Oryza sativa
Microarray analysis techniques
Gene Expression Profiling
Organic Chemistry
Oryza
biology.organism_classification
Actins
Bryopsida
abiotic stresses
Destrin
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
expression pattern
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 5
International Journal of Molecular Sciences, Vol 21, Iss 5, p 1751 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21051751
Popis: Actin-depolymerizing factor (ADF) is a small class of actin-binding proteins that regulates the dynamics of actin in cells. Moreover, it is well known that the plant ADF family plays key roles in growth, development and defense-related functions. Results: Thirteen maize (Zea mays L., ZmADFs) ADF genes were identified using Hidden Markov Model. Phylogenetic analysis indicated that the 36 identified ADF genes in Physcomitrella patens, Arabidopsis thaliana, Oryza sativa japonica, and Zea mays were clustered into five groups. Four pairs of segmental genes were found in the maize ADF gene family. The tissue-specific expression of ZmADFs and OsADFs was analyzed using microarray data obtained from the Maize and Rice eFP Browsers. Five ZmADFs (ZmADF1/2/7/12/13) from group V exhibited specifically high expression in tassel, pollen, and anther. The expression patterns of 13 ZmADFs in seedlings under five abiotic stresses were analyzed using qRT-PCR, and we found that the ADFs mainly responded to heat, salt, drought, and ABA. Conclusions: In our study, we identified ADF genes in maize and analyzed the gene structure and phylogenetic relationships. The results of expression analysis demonstrated that the expression level of ADF genes was diverse in various tissues and different stimuli, including abiotic and phytohormone stresses, indicating their different roles in plant growth, development, and response to external stimulus. This report extends our knowledge to understand the function of ADF genes in maize.
Databáze: OpenAIRE
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