Substituent Position of Iminocyclitols Determines the Potency and Selectivity for Gut Microbial Xenobiotic-Reactivating Enzymes
| Autor: | Hsien-Ya Lin, Lun-Fu Yeh, Shijay Gao, Punsaldulam Dashnyam, Wei-Che Hsieh, Chun-Hung Lin, Chia-Yu Chen, Zhijay Tu |
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| Rok vydání: | 2020 |
| Předmět: |
Clostridium perfringens
Stereochemistry Molecular Conformation Substituent Plasma protein binding Crystallography X-Ray Structure-Activity Relationship chemistry.chemical_compound Bacterial Proteins Piperidines Ruminococcus gnavus Catalytic Domain Drug Discovery Escherichia coli Animals Structure–activity relationship Potency Enzyme Assays Glucuronidase chemistry.chemical_classification Clostridiales Gastrointestinal Microbiome Enzyme chemistry Molecular Medicine Cattle Selectivity Xenobiotic Cyclitols Protein Binding |
| Zdroj: | Journal of Medicinal Chemistry. 63:4617-4627 |
| ISSN: | 1520-4804 0022-2623 |
| Popis: | Selective inhibitors of gut bacterial β-glucuronidases (GUSs) are of particular interest in the prevention of xenobiotic-induced toxicities. This study reports the first structure-activity relationships on potency and selectivity of several iminocyclitols (2-7) for the GUSs. Complex structures of Ruminococcus gnavus GUS with 2-7 explained how charge, conformation, and substituent of iminocyclitols affect their potency and selectivity. N1 of uronic isofagomine (2) made strong electrostatic interactions with two catalytic glutamates of GUSs, resulting in the most potent inhibition (Ki ≥ 11 nM). C6-propyl analogue of 2 (6) displayed 700-fold selectivity for opportunistic bacterial GUSs (Ki = 74 nM for E. coli GUS and 51.8 μM for RgGUS). In comparison with 2, there was 200-fold enhancement in the selectivity, which was attributed to differential interactions between the propyl group and loop 5 residues of the GUSs. The results provide useful insights to develop potent and selective inhibitors for undesired GUSs. |
| Databáze: | OpenAIRE |
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