Glucose-6-Phosphatase Gene Expression and Activity Are Modulated in Hemorrhagic Shock: Evidence for a New Heat-Sensitive Activator
| Autor: | El-Maghrabi, Naji N. Abumrad, S. R. Maitra, A. J. Lange, Wansong Pan, S. J. Pilkis |
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| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty Hot Temperature Biophysics Shock Hemorrhagic Emergency Nursing Biology Biochemistry Gene Expression Regulation Enzymologic Rats Sprague-Dawley Heat sensitive chemistry.chemical_compound Internal medicine Gene expression medicine Animals RNA Messenger Molecular Biology chemistry.chemical_classification Messenger RNA business.industry Activator (genetics) Gluconeogenesis Fructose Cell Biology Molecular biology Rats Enzyme Endocrinology Liver chemistry Hemorrhagic shock Glucose-6-Phosphatase Emergency Medicine Microsome biology.protein Cardiology and Cardiovascular Medicine business Phosphoenolpyruvate carboxykinase Glycolysis Glucose 6-phosphatase |
| Zdroj: | Biochemical and Biophysical Research Communications. 204:716-724 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.2518 |
| Popis: | Decreased hepatic fructose 2,6-bisphosphate levels were observed in the early phase of hemorrhagic shock. The lower sugar bisphosphatae level was a result of increased phosphoenolpyruvate levels and decreased glucose-6-phosphate and fructose-6-phosphate levels. The decreased glucose-6-phosphate levels correlated with increased activity of liver glucose-6-phosphatase and a concomitant 2.5-fold increase in glucose-6-phosphatase mRNA abundance. In addition, protein-free filtrate from hemorrhagic shock rats, but not from control rats, increased glucose-6-phosphatase activity. However, when control and hemorrhagic shock protein-free filtrates were heated, they both increased the glucose-6-phosphatase activity of the respective microsomes to the same extent. It is concluded that the early hyperglycemic phase of hemorrhagic shock is due to enhanced glucose-6-phosphatase gene expression and activity and the generation of a heat sensitive activator of the enzyme. |
| Databáze: | OpenAIRE |
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