MicroRNA-218 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and Accelerates Bone Fracture Healing
| Autor: | Jinfang Zhang, Lu Feng, Weiping Lin, Gang Li, Ji-qiang Duan, Liu Shi, Yang Liu |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Endocrinology Diabetes and Metabolism Bone Marrow Cells Bone healing Marker gene Bone and Bones Mice 03 medical and health sciences Endocrinology Osteogenesis In vivo microRNA Animals Medicine Orthopedics and Sports Medicine Cells Cultured Cell Proliferation Fracture Healing Femur fracture business.industry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells X-Ray Microtomography Immunohistochemistry Mice Inbred C57BL MicroRNAs 030104 developmental biology Cancer research Alkaline phosphatase Stress Mechanical business Femoral Fractures |
| Zdroj: | Calcified Tissue International. 103:227-236 |
| ISSN: | 1432-0827 0171-967X |
| DOI: | 10.1007/s00223-018-0410-8 |
| Popis: | As a regulator of osteogenesis, microRNA-218 (miR-218) is reported to promote osteogenesis of mesenchymal stem cells (MSCs). However, the in vivo osteogenic effect of miR-218 remains elusive. In this study, miR-218 was confirmed to promote osteogenic differentiation of MSCs by stimulating the alkaline phosphatase activity, calcium nodule formation, and osteogenic marker gene expression. For in vivo study, the miR-218-overexpressing BMSCs were locally administrated into the fracture sites in a femur fracture mouse model. Based on the X-rays, micro-computed tomography, mechanical testing, histology, and immunohistochemistry examinations, miR-218 overexpression improved new bone formation and accelerated fracture healing. These findings suggest that miR-218 may be a promising therapeutic target for bone repair in future clinical applications. |
| Databáze: | OpenAIRE |
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