Additional file 2 of From Eat to trEat: engineering the mitochondrial Eat1 enzyme for enhanced ethyl acetate production in Escherichia coli

Autor: Kruis, Aleksander J., Bohnenkamp, Anna C., Nap, Bram, Nielsen, Jochem, Mars, Astrid E., Wijffels, Rene H., Oost, John Van Der, Servé W. M. Kengen, Weusthuis, Ruud A.
Rok vydání: 2020
DOI: 10.6084/m9.figshare.12153978.v1
Popis: Additional file 2: Figure S2. Lack of correlation between ethyl acetate formation and predicted strength of the RBS controlling the production of K. marxianus trEat1. Ethyl acetate titres were obtained from E. coli BW25113 ΔackAΔldhA (DE3) producing K. marxianus trEat1 variants at 0.01 mM IPTG concentration (Fig. 2b). The translation initiation rates of the RBS were predicted with the RBS calculator [22].
Databáze: OpenAIRE