Arabidopsis YELLOW STRIPE-LIKE7 (YSL7) and YSL8 Transporters Mediate Uptake of Pseudomonas Virulence Factor Syringolin A into Plant Cells
| Autor: | Robert Dudler, Silvia Schelbert Hofstetter, Heidi Widmer, Alexey Dudnik |
|---|---|
| Přispěvatelé: | University of Zurich, Dudler, Robert |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Virulence Factors Physiology Recombinant Fusion Proteins Mutant Arabidopsis Gene Expression Pseudomonas syringae Saccharomyces cerevisiae 580 Plants (Botany) Peptides Cyclic Plant Roots 01 natural sciences Virulence factor 03 medical and health sciences 10126 Department of Plant and Microbial Biology Genes Reporter 1102 Agronomy and Crop Science Phylogeny Plant Diseases 030304 developmental biology 0303 health sciences biology Arabidopsis Proteins Pseudomonas Wild type Membrane Transport Proteins Biological Transport 1314 Physiology General Medicine biology.organism_classification Plant cell Yeast Cell biology Plant Leaves Biochemistry Organ Specificity Seedlings Mutation Oligopeptides Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | MOLECULAR PLANT-MICROBE INTERACTIONS |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-06-13-0163-r |
| Popis: | Syringolin A (SylA), a virulence factor secreted by certain strains of the plant pathogen Pseudomonas syringae pv. syringae, is an irreversible proteasome inhibitor imported by plant cells by an unknown transport process. Here, we report that functional expression in yeast of all 17 members of the Arabidopsis oligopeptide transporter family revealed that OLIGOPEPTIDE TRANSPORTER1 (OPT1), OPT2, YELLOW STRIPE-LIKE3 (YSL3), YSL7, and YSL8 rendered yeast cells sensitive to growth inhibition by SylA to different degrees, strongly indicating that these proteins mediated SylA uptake into yeast cells. The greatest SylA sensitivity was conferred by YSL7 and YSL8 expression. An Arabidopsis ysl7 mutant exhibited strongly reduced SylA sensitivity in a root growth inhibition assay and in leaves of ysl7 and ysl8 mutants, SylA-mediated quenching of salicylic-acid-triggered PATHOGENESIS-RELATED GENE1 transcript accumulation was greatly reduced compared with the wild type. These results suggest that YSL7 and YSL8 are major SylA uptake transporters in Arabidopsis. Expression of a YSL homolog of bean, the host of the SylA-producing P. syringae pv. syringae B728a, in yeast also conferred strong SylA sensitivity. Thus, YSL transporters, which are thought to be involved in metal homeostasis, have been hijacked by bacterial pathogens for SylA uptake into host cells. |
| Databáze: | OpenAIRE |
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