Induction of enhanced green fluorescent protein expression in response to lesions in the nervous system

Autor: Andre Stutz, Lorenza Eder-Colli, Michel Dubois-Dauphin, Serge Nef, Jean-Dominique Vassalli, Philippe G. Vallet
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Untranslated region
Time Factors
ddc:616.07
Nervous System
Functional Laterality
Green fluorescent protein
In Situ Hybridization/methods
Mice
Tubulin
Untranslated Regions
ddc:576.5
RNA
Messenger/physiology
Stroke/etiology/metabolism
Promoter Regions
Genetic
In Situ Hybridization
Nervous System/metabolism/pathology
General Neuroscience
Brain
Infarction
Middle Cerebral Artery
Glial Fibrillary Acidic Protein/metabolism
Brain/anatomy & histology/metabolism
Stroke
Tissue Plasminogen Activator
Tissue Plasminogen Activator/physiology
Genetically modified mouse
Facial Nerve Injuries/metabolism
Transgene
Green Fluorescent Proteins
Mice
Transgenic
In situ hybridization
Biology
Glial Fibrillary Acidic Protein
Gene silencing
Animals
Humans
RNA
Messenger
Ubiquitins
Facial Nerve Injuries
Messenger RNA
Murine hepatitis virus
Three prime untranslated region
Tubulin/metabolism
Ubiquitins/genetics
Molecular biology
Promoter Regions
Genetic/genetics
Luminescent Proteins/metabolism
Luminescent Proteins
Infarction
Middle Cerebral Artery/complications/metabolism
Gene Expression Regulation
Microscopy
Fluorescence
Zdroj: Journal of Comparative Neurology, Vol. 474, No 1 (2004) pp. 108-22
ISSN: 0021-9967
Popis: We have generated a mouse strain carrying a transgene driven by a strong and ubiquitous promoter (human cytomegalovirus hCMV/beta-actin) and containing an enhanced green fluorescent protein (eGFP) coding sequence upstream of the 3' untranslated region (3'UTR) of tissue-type plasminogen activator (t-PA) mRNA. The 3'UTR of t-PA mRNA is known to be involved in the reversible deadenylation and translational repression of transcripts in mouse oocytes. hCMV/beta-actin-eGFP-3'UTR t-PA transgenic mice express eGFP mRNA in all brain structures analyzed but lack eGFP fluorescence, with the exception of blood vessels, choroid plexus, and Purkinje cells. Taking advantage of these features, we tested whether certain pathological conditions, in particular injuries of the nervous system, might trigger eGFP fluorescence in traumatized cells or neurons. From this perspective, we analyzed eGFP mRNA expression and eGFP fluorescence in experimental models of nervous system lesions, such as motoneuron axotomy and cerebral stroke induced by middle cerebral artery occlusion. We found an increase in eGFP fluorescence in specific brain areas in cells suffering or reacting to these injuries. This increased fluorescence is correlated with an increased transcription of eGFP in lesioned cells, presumably enhanced by a release of the translational silencing mediated by the 3'UTR region of the t-PA mRNA. This transgenic mouse model may prove useful to study the development of neurodegenerative lesions.
Databáze: OpenAIRE
Externí odkaz: