Antrodia cinnamomea mycelial fermentation broth inhibits the epithelial-mesenchymal transition of human esophageal adenocarcinoma cancer cells
| Autor: | Yu-Kuo Liu, Yu-Jen Chen, Pin-I Huang, Yu Ming Liu, Tung Hu Tsai |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Epithelial-Mesenchymal Transition Esophageal Neoplasms Down-Regulation Vimentin Adenocarcinoma Toxicology Metastasis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Transforming Growth Factor beta Cell Line Tumor medicine Humans Epithelial–mesenchymal transition Biological Products Mycelium biology Chemistry Twist-Related Protein 1 Nuclear Proteins General Medicine Esophageal cancer Cadherins medicine.disease Culture Media 030104 developmental biology 030220 oncology & carcinogenesis Antrodia Fermentation Cancer cell Cancer research biology.protein Antrodia cinnamomea Signal Transduction Food Science Transforming growth factor |
| Zdroj: | Food and Chemical Toxicology. 119:380-386 |
| ISSN: | 0278-6915 |
| Popis: | Esophageal cancer is associated with a high mortality rate and easy metastasis. The aim of this study is to investigate the effect of the bio-product Antrodia cinnamomea mycelial fermentation broth (AC-MFB) on the epithelial mesenchymal transition (EMT) of human esophageal cancer cells and the molecular mechanisms underlying these effects. Transforming growth factor β (TGF-β) was used to induce EMT in human esophageal BE3 cancer cells. Changes in cell morphology and migration potential were examined. The expression of E-cadherin, N-cadherin, vimentin, and other transcriptional factors was studied by western blot assay. The results showed that AC-MFB was not only able to upregulate the expression of Ecadherin and attenuate the TGF-β-induced overexpression of vimentin and N-cadherin, but it also reversed the TGF-β-induced changes in cell morphology from polygonal to spindle-shaped and delayed the migration potential of BE3 cells. Furthermore, AC-MFB treatment was able to inhibit the expression levels of both Twist and Twist1. Overall, AC-MFB was able to inhibit the EMT of esophageal cancer BE3 cells, which was accompanied by Twist and Twist1 downregulation. |
| Databáze: | OpenAIRE |
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