Identification and characterization of a cDNA and the gene encoding the mouse ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein
| Autor: | Richard M. O'Brien, Cyrus C. Martin, Jared N. Boustead, Christina A. Svitek, John C. Hutton, James K. Oeser, SI Hunter |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary Protein subunit Molecular Sequence Data Glucose-6-Phosphate Biology Primer extension Chromosomes Fusion gene Exon Islets of Langerhans Mice Endocrinology Complementary DNA Catalytic Domain Chlorocebus aethiops Animals Humans Tissue Distribution Amino Acid Sequence Cloning Molecular Molecular Biology Gene Cells Cultured Muscles Promoter Molecular biology Open reading frame Liver COS Cells Glucose-6-Phosphatase Sequence Alignment HeLa Cells |
| Zdroj: | Journal of molecular endocrinology. 32(1) |
| ISSN: | 0952-5041 |
| Popis: | Glucose-6-phosphatase (G6Pase) catalyzes the final step in the gluconeogenic and glycogenolytic pathways, the hydrolysis of glucose-6-phosphate (G6P) to glucose and phosphate. This paper describes the identification and characterization of a cDNA and the gene encoding the mouse ubiquitously expressed G6Pase catalytic subunit-related protein (UGRP). The open reading frame of this UGRP cDNA encodes a protein (346 amino acids (aa); Mr 38,755) that shares 36% overall identity (56% similarity) with the mouse G6Pase catalytic subunit (357 aa; Mr 40,454). UGRP exhibits a similar predicted transmembrane topology and conservation of many of the catalytically important residues with the G6Pase catalytic subunit; however, unlike the G6Pase catalytic subunit, UGRP does not catalyze G6P hydrolysis and does not contain a carboxy-terminal di-lysine endoplasmic reticulum retention signal. UGRP mRNA was detected by RNA blot analysis in every mouse tissue examined with the highest expression in heart, brain, testis and kidney. Database analysis showed that the mouse UGRP gene is composed of six exons, spans approximately 4.2 kbp of genomic DNA and is located on chromosome 11 along with the G6Pase catalytic subunit gene. The UGRP gene transcription start sites were mapped by primer extension analysis, and the activity of the mouse UGRP gene promoter was analyzed using luciferase fusion gene constructs. In contrast to the G6Pase catalytic subunit gene promoter, the UGRP promoter was highly active in all cell lines examined. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|