Iron oxide nanomatrix facilitating metal ionization in matrix-assisted laser desorption/ionization mass spectrometry
Autor: | I-Che Li, Ying-Wei Lu, Chung-Chen Lin, Florian R. del Mundo, Guillermo Nuesca, Rofeamor P. Obena, Po-Chiao Lin, Yu-Ju Chen, Susan D. Arco |
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Rok vydání: | 2011 |
Předmět: |
Ions
Matrix-assisted laser desorption electrospray ionization Chemistry Analytical chemistry Thermal ionization Atmospheric-pressure chemical ionization Fresh Water Mass spectrometry Ferric Compounds Ion source Analytical Chemistry Atmospheric-pressure laser ionization Metals Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Direct electron ionization liquid chromatography–mass spectrometry interface Magnetite Nanoparticles Electron ionization |
Zdroj: | Analytical chemistry. 83(24) |
ISSN: | 1520-6882 |
Popis: | The significance and epidemiological effects of metals to life necessitate the development of direct, efficient, and rapid method of analysis. Taking advantage of its simple, fast, and high-throughput features, we present a novel approach to metal ion detection by matrix-functionalized magnetic nanoparticle (matrix@MNP)-assisted MALDI-MS. Utilizing 21 biologically and environmentally relevant metal ion solutions, the performance of core and matrix@MNP against conventional matrixes in MALDI-MS and laser desorption ionization (LDI) MS were systemically tested to evaluate the versatility of matrix@MNP as ionization element. The matrix@MNPs provided 20- to100-fold enhancement on detection sensitivity of metal ions and unambiguous identification through characteristic isotope patterns and accurate mass (5 ppm), which may be attributed to its multifunctional role as metal chelator, preconcentrator, absorber, and reservoir of energy. Together with the comparison on the ionization behaviors of various metals having different ionization potentials (IP), we formulated a metal ionization mechanism model, alluding to the role of exciton pooling in matrix@MNP-assisted MALDI-MS. Moreover, the detection of Cu in spiked tap water demonstrated the practicability of this new approach as an efficient and direct alternative tool for fast, sensitive, and accurate determination of trace metal ions in real samples. |
Databáze: | OpenAIRE |
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